Method for manufacturing personalized nutritional compound composition based on gene polymorphism
A gene polymorphism and composition technology, which is applied in biochemical equipment and methods, microbial determination/inspection, food science, etc., can solve problems such as reducing the efficiency and willingness of use, inconvenience in taking, and users forgetting to take it. , to achieve the effect of reducing the possibility of taking the wrong quantity or frequency, reducing the frequency and volume and quality, and increasing the efficiency and willingness of use
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[0034] Obtain nucleic acid-containing specimen samples from voluntary subjects, using the present invention using TaqMan technology ( SNP Genotyping Assays, purchased from Applied Biosystems Inc.) to identify genotypes of single nucleotide polymorphisms. This technology is to design two probes for a SNP, design two probes with different sequences according to the normal genotype (Wild-Type) and mutant genotype (Mutant-Type) of the SNP, and pair them specifically to different two alleles. The 5' ends of the probes are labeled with different fluorescent colors, which are called reporter dyes (Reporter Dye). The fluorescent colors used for labeling are usually FAMTM dye (FAMTM dye) and VICTM dye (VICTM dye), but other fluorescent dyes can also be used systems, such as TET dyes (TET dye). The 3' end of the probe is labeled with a non-fluorescent substance that can absorb fluorescent energy, called a quencher dye (Quancher Dye), and the substance used for the label is (tetrameth...
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