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Tissue culture and rapid propagation technology for ulmus pumila Linn.

A technology of tissue culture rapid propagation and technology, applied in the field of plant tissue culture, can solve the problems of limited number and speed of reproduction, the tissue culture system of elm tree is not fully mature, and the difficulty of rooting cuttings, etc., to achieve the effect of rapid propagation

Inactive Publication Date: 2015-06-03
陈凤佳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is difficult for cuttings to take root, grafting will be limited by the number of scions, and there are technical requirements, which directly lead to a great limitation in the number and speed of reproduction
In recent years, regenerated plants of some varieties of elm have been obtained by induction of different explants, but the tissue culture system of elm is not fully mature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection and processing of explants: Pick the shoots in seasons when the plants grow vigorously and the temperature is relatively cool, wrap the incisions with moist absorbent cotton, and store them in a -4°C refrigerator for later use. Cut the axillary buds of the stem into about 2-3 cm long, remove the leaves and terminal buds, keep a little petiole, soak in washing powder water for 10 minutes, rinse with tap water for 1 hour after brushing, then disinfect with 75% ethanol for 5 seconds, and then wash 3 times with sterile water. Then disinfect with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water for 4 times, and absorb the water with sterile filter paper.

[0018] (2) Primary culture: Inoculate the stem segment from step (1) on the primary culture medium, culture it in total darkness at 25°C for 15 days, then place it under the condition of 12 hours of light per day, and cultivate it for 28 days at a light intensity of 1500 lx. That ...

Embodiment 2

[0023] (1) Collection and processing of explants: Pick the shoots in seasons when the plants grow vigorously and the temperature is relatively cool, wrap the incisions with moist absorbent cotton, and store them in a -4°C refrigerator for later use. Cut the axillary buds of the stem segment into about 2-3 cm long, remove the leaves and terminal buds, keep a little petiole, soak in washing powder water for 12 minutes, rinse with tap water for 2 hours after brushing, disinfect with 75% ethanol for 8 seconds, and wash 5 times with sterile water. Then sterilize with 0.1% mercuric chloride solution for 13 minutes, rinse with sterile water for 5 times, and absorb the water with sterile filter paper.

[0024] (2) Primary culture: inoculate the stem segment from step (1) on the primary culture medium, culture it in total darkness at 27°C for 17 days, then place it in the light for 14 hours a day, and cultivate it for 26 days at a light intensity of 2000 lx. That is to induce the f...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation technology for ulmus pumila Linn., and relates to a propagation method for quickly gaining property-stabilized seedlings of ulmus pumilla Linn. by the rapid clonal propagation technology. The technology comprises the processes of disinfecting of explants, primary culture, propagation culture, rooting culture, hardening-seedling and transplanting and the like. With the adoption of the technology, the rapid propagation of seedlings of ulmus pumilla Linn. is achieved, and basic materials are provided for crop improvement in genetic engineering of ulmus pumila Linn. and industrial seedling culture in the future.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a rapid propagation technique for tissue culture of Ulmus japonica. Background technique [0002] elm ( Ulmus pumila Linn.) is a deciduous tree of the genus Ulmus in the family Ulmaceae. It is light-loving, cold-resistant, drought-resistant, barren-resistant, and it does not choose the soil and has strong adaptability. The root system is relatively developed, strong in wind resistance and soil retention; strong in germination, resistant to pruning; fast in growth and long in life. It is an excellent tree species for greening barren mountains and maintaining water and soil. At the same time, it is also the main tree species for urban greening, shelterbelts, and saline-alkali land improvement. It can be planted alone, and can also be used as street trees, forest protection belts, etc. At the same time, it has strong adaptability, easy managemen...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G17/00
Inventor 陈凤佳
Owner 陈凤佳
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