Tissue culture type rhizoma gastrodiae breeding technology

A technology of Gastrodia elata and tissue culture, applied in the field of tissue culture Gastrodia elata breeding, can solve the problems of thin and small seeds, insufficient nutrition, absorption of nutrients, etc., and achieve the effects of simple technical steps, pure quality of Gastrodia elata, and high germination rate.

Inactive Publication Date: 2017-12-08
LIUPANSHUI QIANFENG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gastrodia elata is a perennial herbaceous parasite of Orchidaceae. It has no roots and no leaves. The plant does not contain chlorophyll, so it cannot carry out photosynthesis and is self-supporting. It cannot directly absorb nutrients from the outside. Bacteria provide Gastrodia elata with the nutrients needed for growth. The combination rate of protocorms of Gastrodia elata seeds and Armillaria mellea is not high in conventional methods, and they are easily infected with bacteria or miscellaneous bacteria, resulting in reduced or no harvest; vulnerable to pests, rodents, and other animal hazards; cultivated The risk of diseased and residual seeds is very high, which will easily lead to reduced yield or no harvest in the next planting; under the conventional method, the nutrition in the culture material of Armillaria armillaria is not enough, and the individual seeds produced are thin and small, which is inconvenient to manage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of tissue culture gastrodia elata breeding technology, comprises the following steps:

[0021] S1) Mushroom material preparation: first select elm, locust tree or birch to cut off the branches, and then cut the branches into small sections with a knife to process the fungus branches, or saw elm, locust tree or birch stems into 1.0 feet long segment, the side of the segment is evenly cut into 4 rows of fish scale mouths, and the distance between the upper and lower fish scale mouths is 2.0 inches, so as to intercept the fungus sticks. When selecting fungus branches, keep the length of the fungus branches at 8 cm, and the selected The branch diameter is 2 cm;

[0022] S2) Bacterial material treatment: Soak the branches and sticks in nutrient solution for 1 hour and take them out, then air-dry the surface of the branches and sticks. The nutrient solution is prepared from water, ammonium nitrate and magnesium sulfate. The mass ratio of magnesium is 100:1:1;

[0023...

Embodiment 2

[0030] A kind of tissue culture gastrodia elata breeding technology, comprises the following steps:

[0031] S1) Mushroom material preparation: first select elm, locust tree or birch to cut off the branches, and then cut the branches into small sections with a knife to process the fungus branches, or saw elm, locust tree or birch poles into 1.2 feet long segment, the side of the segment is evenly cut into 4 rows of fish scale mouths, and the distance between the upper and lower fish scale mouths is 2.3 inches, so as to intercept the fungus sticks. When selecting fungus branches, keep the length of the fungus branches at 9 cm, and the selected The branch diameter is 1.2 cm;

[0032] S2) Bacteria treatment: Soak the branches and sticks in nutrient solution for 1.3 hours and take them out, then air-dry the surface of the branches and sticks. The nutrient solution is prepared from water, ammonium nitrate and magnesium sulfate. Water, ammonium nitrate and sulfuric acid The mass ra...

Embodiment 3

[0040] A kind of tissue culture gastrodia elata breeding technology, comprises the following steps:

[0041] S1) Mushroom material preparation: first select elm, locust tree or birch to cut off the branches, and then cut the branches into small sections with a knife to process the fungus branches, or saw elm, locust tree or birch poles into 1.4 feet long segment, the side of the segment is evenly cut into 4 rows of fish scale mouths, and the distance between the upper and lower fish scale mouths is 2.8 inches, so as to intercept the fungus sticks. When selecting the fungus branches, keep the length of the fungus branches at 10 cm, and the selected The branch diameter is 2.8 cm;

[0042] S2) Bacteria treatment: Soak the branches and sticks in nutrient solution for 1.8 hours and take them out, then air-dry the surface of the branches and sticks. The nutrient solution is prepared from water, ammonium nitrate and magnesium sulfate. Water, ammonium nitrate and sulfuric acid The ma...

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Abstract

The invention provides a tissue culture type rhizoma gastrodiae breeding technology. The technology includes steps of selecting the cut branches of elm, locust tree or birch; cutting branches to be small section by a cutter so as to process a bacteria branch; or sawing the elm, locust tree or birch to be sections with length of 1.0-1.5 cun; evenly cutting the side faces of the sections to be four lines of scale openings, wherein the space between upper and lower scale openings is 2.0-3.0 cun; intercepting a bacteria rod, and soaking the bacteria branch and the bacteria rod in the nutrition liquid and then taking out; air-drying the surfaces of the bacteria branch and the bacteria rod; selecting the seed stems with complete individual development, scab-free, distortion-free, normal gloss and obvious eye; soaking the bacterial materials sin the nutrition fluid in the course of planting so that the germination rate of rhizoma gastrodiae is higher. For the soil is mixed with the nutrition fluid, the rhizoma gastrodiae is grown better by comparing with the process of adding the nutrition fluid in the potato water. The tissue culture type rhizoma gastrodiae breeding technology is simple in steps, and the breed rhizoma gastrodiae quality is more purified.

Description

technical field [0001] The invention relates to the technical field of gastrodia elata breeding, in particular to a tissue culture gastrodia elata breeding technology. Background technique [0002] Gastrodia elata, also known as Chijian, Dingfengcao, Limu, Bailongpi, and Tomorrow Ma, is a precious Chinese herbal medicine. Gastrodia elata is sweet, pungent, flat, non-toxic, and belongs to the liver meridian. It can relieve wind and spasm, calm liver yang, dispel wind and dredge collaterals. It can treat convulsions, convulsions, dizziness, headache, hemiplegia, numbness of limbs, rheumatic arthralgia and other effects. Gastrodia elata is a perennial herbaceous parasite of Orchidaceae. It has no roots and no leaves. The plant does not contain chlorophyll, so it cannot carry out photosynthesis and is self-supporting. It cannot directly absorb nutrients from the outside. Bacteria provide Gastrodia elata with the nutrients needed for growth. The combination rate of protocorms o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C05G3/00
CPCA01G31/00A01G24/23C05C1/00C05G5/20C05D5/00
Inventor 周明辉曹强
Owner LIUPANSHUI QIANFENG AGRI DEV CO LTD
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