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Fibroblast growth factor inhibitory polypeptide and application thereof

A technology of fibroblasts and growth factors, applied in the field of polypeptides for the treatment of malignant tumors, to achieve the effect of improving the survival rate

Inactive Publication Date: 2015-06-10
SUZHOU PULUODA BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no maturely developed fibroblast growth factor inhibitory polypeptide available for the treatment of malignant tumors

Method used

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  • Fibroblast growth factor inhibitory polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Fibroblast Growth Factor Inhibitory Peptide IC50 on Growth and Survival of Human Colon Cancer Cells Cultured in Vitro.

[0028] The MTT colorimetric method was used. The logarithmic growth of human colon cancer cells was added to a 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug fibroblast growth factor inhibitory polypeptide (Shanghai Sangong Synthetic Co., Ltd.) were added to the experimental wells and positive drug control wells respectively. ) and the positive control drug paclitaxel; the same volume of solvent was added to the blank group. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and incubate at 620nm in a microplate reader The absorbance A value was measured, according to the formula human colon cancer cell growth inhibition rate=(1-absorbance value of the experi...

Embodiment 2

[0030] Fibroblast Growth Factor Inhibitory Peptide IC50 on Growth and Survival of Human Hepatoma Cells Cultured in Vitro.

[0031] The MTT colorimetric method was used. The logarithmic growth of human liver cancer HepG2 cells was added to a 96-well culture plate at a volume of 1.0×105, and cultured for 24 hours. Different concentrations of the experimental drug fibroblast growth factor inhibitory polypeptide and the positive control drug paclitaxel were added to the experimental wells and positive drug control wells, respectively. ; Add the same volume of solvent to the blank group. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and incubate at 620nm in a microplate reader The absorbance A value was measured, according to the formula human HepG2 cell growth inhibition rate=(1-absorbance value of the experimental group / absorbance value of the c...

Embodiment 3

[0033] The in vivo activity of fibroblast growth factor inhibitory polypeptide was detected by tumor model.

[0034] The colon cancer tumor model was established, and paclitaxel was used as a positive control drug; the same volume of solvent was added to the blank group, and three doses of polypeptide (Shanghai Sangong Synthetic) were set in the experimental group: 1, 2, and 4 mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results show that fibroblast growth factor inhibitory polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice. The survival rates of 0.75, 1.5, and 3 mg / Kg reached 92.12%, 82.23%, and 72.4%, and the corresponding paclitaxel was 42.2%. .

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Abstract

The invention discloses fibroblast growth factor inhibitory polypeptide and an application thereof, and relates to the field of drugs, in particular to the polypeptide capable of restraining fibroblast growth factors and treating malignant tumors. The sequence is PSHNPEEQLSWGDSGEDSEPLP which is a branch-new sequence. The inhibitory polypeptide can restrain the proliferation of colonic cancer cells and hepatoma cells in vitro, the survival rate of tumor-bearing mice can be increased in vivo tests, and potential development value of new drugs can be achieved.

Description

technical field [0001] The invention relates to fibroblast growth factor inhibiting polypeptide and application thereof, in particular to a polypeptide capable of inhibiting fibroblast growth factor and treating malignant tumors. Background technique [0002] Angiogenesis is required for the further growth of malignant solid tumors beyond the epithelial basement membrane. The newly formed blood vessels in the tumor have their own unique structural characteristics, which are characterized by incomplete walls, no smooth muscle components, and only composed of porous endothelial cells and sheet-like basement membrane. Most scholars believe that the structural characteristics of new blood vessels make it possible for distant metastasis of malignant tumor tissue. Therefore, quantification of neovascularization in malignant solid tumors is considered to be an important independent prognostic marker. [0003] Fibroblast growth factor has obvious tropism activity on tumor cells, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K38/16A61P35/00G01N33/68G01N33/574
CPCC07K14/00A61K38/00G01N33/574G01N33/68G01N2333/50
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
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