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Yeast recovery technology in ultrahigh-concentration ethanol fermentation process

A technology of ethanol fermentation and concentration, applied in fermentation and other directions, can solve the problem of no in-depth discussion on the reuse of yeast surfactants, and achieve the effects of protecting the survival rate of yeast cells, reducing production costs, and improving fermentation efficiency.

Inactive Publication Date: 2015-06-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recovery of yeast and the reuse of surfactants have not been discussed in depth.

Method used

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  • Yeast recovery technology in ultrahigh-concentration ethanol fermentation process
  • Yeast recovery technology in ultrahigh-concentration ethanol fermentation process
  • Yeast recovery technology in ultrahigh-concentration ethanol fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water at 25-38°C for 15-30 minutes, then add 6.5g of glucose and 12mL of pre-configured sulfuric acid solution with a pH value of 4.8, and place it in a shaker for shaking. Ferment at 33°C for 48-72 hours. After the fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove the clear night from the fermentation broth after the fermentation, leave solid yeast powder, add 4mL ultrapure water, 6.5g glucose, 12mL sulfuric acid solution, and ferment at 33°C for 48-72 hours, so Cycle 2 times. Glucose conversion rate, ethanol yield and concentration are as follows image 3 , Figure 4 , Figure 5 shown. It can be seen from the data in the figure that when no surfactant is added to the fermentation system, the recovery efficiency of yeast is very low. After 48 hours of fermentation, the recovered yeast can only be recycled for the first time to obtain 60g.L -1 ethanol concentration.

Embodiment 2

[0028] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water for 15-30 minutes at 25-38°C, then add 6.5g of glucose, PEG-4004.0g, and 12mL of a pre-prepared sulfuric acid solution with a pH value of 4.8, and put it in a shaker Shake in the bed, and ferment for 72 hours at 33°C. After the fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove the clear night from the fermentation broth after the fermentation, leave solid yeast powder, add 4mL ultrapure water, 6.5g glucose, 4.0g PEG-400, 12mL sulfuric acid solution, and ferment at 33°C 48 hours, so cycle 3 times. Glucose conversion rate, ethanol yield and concentration are as follows Figure 6 , Figure 7 , Figure 8 shown. It can be seen from the data in the figure that when PEG-400 is added to the fermentation system, the yeast recovery efficiency is very high. After 48 hours of fermentation, the recovered yeast can be recycled for the first time to obtain 118g.L -1 ethanol concentration.

Embodiment 3

[0030] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water for 15-30 minutes at 25-38°C, then add 7.5g of glucose, 4.0g of PEG-400, and 12mL of pre-configured sulfuric acid solution with a pH value of 4.8, and put Vibrate in a shaker, and ferment for 72 hours at 33°C. After fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove solid yeast powder, distill the supernatant, and the residue after distillation enters the next fermentation process, add 0.5g yeast powder again and use 4mL ultrapure water Rehydrate for 15-30 minutes, 6.5g glucose, 12mL sulfuric acid solution, ferment at 33°C for 72 hours, and cycle like this 4 times. Glucose conversion rate, ethanol yield and concentration such as Figure 9 It was shown that the surfactant PEG-400 could be recycled and had no significant effect on the fermentation efficiency.

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Abstract

The invention discloses a yeast recovery technology in an ultrahigh-concentration ethanol fermentation process, which realizes long service life of the yeast and recovery utilization. Surfactants are added into a fermentation culture medium and are used as protective agents for yeast cells, so that the service life of the yeast cell is greatly improved and the survival rate in ultrahigh-concentration ethanol fermentation liquid is increased so that the yeast can be recycled. The technology comprises the steps that yeast powder, glucose, surfactants and sulfuric acid solutions are mixed and fermented, and the yeast and the surfactants are recovered by means of freezing centrifugal separation and pressure reduction distillation after the fermentation is completed and are used in a new fermentation process. According to the yeast recovery technology, the cyclic utilization of the yeast is improved, and the synchronous recycling and reusing of the surfactants and sulfuric acid is realized; the fermentation process is simple, the production cost of ethanol fuels can be effectively reduced, the fermentation efficiency is improved, and the technology has important application prospect.

Description

technical field [0001] The invention relates to the field of ethanol production by a fermentation process, in particular to a yeast recovery technology in an ultra-high-concentration ethanol fermentation process. Background technique [0002] With the development of the world economy, especially the development of the automobile industry, the supply of oil is in short supply, and governments of various countries have begun to consider the issue of fuel self-sufficiency. The emergence of the fuel ethanol industry has effectively alleviated the shortage of petroleum resources and reduced dependence on petroleum; it is also conducive to the improvement of the environment. The use of ethanol gasoline for vehicles can reduce the level of vehicle exhaust pollution by more than 30% on average. Therefore, fuel ethanol is a kind of "green energy" that can effectively alleviate the energy crisis and open up a new way to maintain sustainable economic and social development. [0003] P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/14
CPCC12P7/14
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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