A kind of yeast recovery technology in ultra-high concentration ethanol fermentation process

A technology of ethanol fermentation and concentration, applied in fermentation and other directions, can solve the problem that the reuse of yeast surfactants has not been discussed in depth, and achieve the effects of protecting the survival rate of yeast cells, reducing production costs, and improving fermentation efficiency.

Inactive Publication Date: 2018-06-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recovery of yeast and the reuse of surfactants have not been discussed in depth.

Method used

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  • A kind of yeast recovery technology in ultra-high concentration ethanol fermentation process
  • A kind of yeast recovery technology in ultra-high concentration ethanol fermentation process
  • A kind of yeast recovery technology in ultra-high concentration ethanol fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water at 25-38°C for 15-30 minutes, then add 6.5g of glucose and 12mL of pre-configured sulfuric acid solution with a pH value of 4.8, and place it in a shaker for shaking. Ferment at 33°C for 48-72 hours. After the fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove the clear night from the fermentation broth after the fermentation, leave solid yeast powder, add 4mL ultrapure water, 6.5g glucose, 12mL sulfuric acid solution, and ferment at 33°C for 48-72 hours, so Cycle 2 times. Glucose conversion rate, ethanol yield and concentration are as follows image 3 , Figure 4 , Figure 5 shown. It can be seen from the data in the figure that when no surfactant is added to the fermentation system, the recovery efficiency of yeast is very low. After 48 hours of fermentation, the recovered yeast can only be recycled for the first time to obtain 60g.L -1 ethanol concentration.

Embodiment 2

[0028] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water for 15-30 minutes at 25-38°C, then add 6.5g of glucose, PEG-4004.0g, and 12mL of a pre-prepared sulfuric acid solution with a pH value of 4.8, and put it in a shaker Shake in the bed, and ferment for 72 hours at 33°C. After the fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove the clear night from the fermentation broth after the fermentation, leave solid yeast powder, add 4mL ultrapure water, 6.5g glucose, 4.0g PEG-400, 12mL sulfuric acid solution, and ferment at 33°C 48 hours, so cycle 3 times. Glucose conversion rate, ethanol yield and concentration are as follows Figure 6 , Figure 7 , Figure 8 shown. It can be seen from the data in the figure that when PEG-400 is added to the fermentation system, the yeast recovery efficiency is very high. After 48 hours of fermentation, the recovered yeast can be recycled for the first time to obtain 118g.L -1 ethanol concentration.

Embodiment 3

[0030] Rehydrate 0.5g of yeast powder with 4mL of ultrapure water for 15-30 minutes at 25-38°C, then add 7.5g of glucose, 4.0g of PEG-400, and 12mL of pre-configured sulfuric acid solution with a pH value of 4.8, and put Vibrate in a shaker, and ferment for 72 hours at 33°C. After fermentation, refrigerate and centrifuge at 8000rpm for 5 minutes, remove solid yeast powder, distill the supernatant, and the residue after distillation enters the next fermentation process, add 0.5g yeast powder again and use 4mL ultrapure water Rehydrate for 15-30 minutes, 6.5g glucose, 12mL sulfuric acid solution, ferment at 33°C for 72 hours, and cycle like this 4 times. Glucose conversion rate, ethanol yield and concentration such as Figure 9 It was shown that the surfactant PEG-400 could be recycled and had no significant effect on the fermentation efficiency.

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Abstract

A yeast recovery technology in the ultra-high concentration ethanol fermentation process, which prolongs the life of yeast and makes it recyclable. By adding a surfactant in the fermentation medium as a protective agent for yeast cells, the life span of the yeast cells and the survival rate in the ultra-high concentration ethanol fermentation broth are greatly improved, making it recyclable. First, yeast powder, glucose, surfactant, and sulfuric acid solution are mixed for fermentation. After the fermentation, the yeast and surfactant are recovered by refrigerated centrifugation and vacuum distillation respectively for a new fermentation process. The invention not only improves the recycling efficiency of yeast, but also realizes synchronous recovery and reuse of surfactant and sulfuric acid, has a simple fermentation process, can effectively reduce fuel ethanol production cost, and improves fermentation efficiency, and has important application prospects.

Description

technical field [0001] The invention relates to the field of ethanol production by a fermentation process, in particular to a yeast recovery technology in an ultra-high-concentration ethanol fermentation process. Background technique [0002] With the development of the world economy, especially the development of the automobile industry, the supply of oil is in short supply, and governments of various countries have begun to consider the issue of fuel self-sufficiency. The emergence of the fuel ethanol industry has effectively alleviated the shortage of petroleum resources and reduced dependence on petroleum; it is also conducive to the improvement of the environment. The use of ethanol gasoline for vehicles can reduce the level of vehicle exhaust pollution by more than 30% on average. Therefore, fuel ethanol is a kind of "green energy" that can effectively alleviate the energy crisis and open up a new way to maintain sustainable economic and social development. [0003] P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/14
CPCC12P7/14
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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