A kind of surfactant improves the method for yeast cell toxin-resistant fermentation performance

A technology of surfactants and yeast cells, which is applied in the field of fuel ethanol production through fermentation process through the degradation of biomass raw materials, to achieve the effects of improving fermentation efficiency, good application prospects, and protecting survival rate

Active Publication Date: 2019-05-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for improving the toxicity-resistant fermentation performance of yeast cells with surfactants aimed at the inhibitory effect of toxic substances produced in the existing biomass pretreatment process on the subsequent fermentation process

Method used

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  • A kind of surfactant improves the method for yeast cell toxin-resistant fermentation performance
  • A kind of surfactant improves the method for yeast cell toxin-resistant fermentation performance
  • A kind of surfactant improves the method for yeast cell toxin-resistant fermentation performance

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose and furfural (0-5g.L -1 ), 6 mL of a pre-configured sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 72 hours.

[0046] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose and furfural (0-5g.L -1 ), PEG-400 (2.0g), pre-configured 6mL sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 72 hours.

[0047] Glucose conversion rate, ethanol yield and concentration are as follows figure 1 , figure 2 , image 3 shown. It can be seen from the data in the figure that adding furfural to the fermentation system has a strong inhibitory effect on the fermentation efficiency, and the fermentation efficiency gradually decreases with the increase of furfural concentration; and when PEG-400 is added to the...

Embodiment 2

[0049] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose and furfural (5g.L -1 ), 6 mL of a pre-configured sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 100 hours.

[0050] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose and furfural (5g.L -1 ), PEG-400 (2.0g), 6mL of a pre-configured sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 100 hours.

[0051] Glucose conversion rate, ethanol yield and concentration are as follows Figure 4 , Figure 5 , Figure 6 shown. As can be seen from the data in the figure, adding furfural (5g.L -1 ) has a strong inhibitory effect on the fermentation efficiency; and when PEG-400 is added to the fermentation system containing furfural, the fermentation efficiency can be significantly improved after the fer...

Embodiment 3

[0053] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose, 5-hydroxymethylfurfural (0-5g.L -1 ), 6 mL of a pre-configured sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 72 hours.

[0054] Rehydrate 0.25g of yeast powder with 2mL of ultrapure water for 15-30 minutes at 33°C, then add 3.5g of glucose, 5-hydroxymethylfurfural (0-5g.L -1 ), PEG-400 (2.0g), 6mL of a pre-configured sulfuric acid solution with a pH value of 4.3, placed in a shaker for shaking, and fermented at 33°C for 72 hours.

[0055] Glucose conversion rate, ethanol yield and concentration are as follows Figure 7 , Figure 8 , Figure 9 shown. It can be seen from the data in the figure that adding 5-hydroxymethylfurfural to the fermentation system has no obvious inhibitory effect on the fermentation efficiency; and when PEG-400 is added to the fermentation system containing 5-hydroxymethylfu...

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Abstract

The invention discloses a method for improving yeast cell toxicity-resistant fermentation performance by a surfactant, and relates to the field of producing fuel ethanol through a fermentation process through the degradation of biomass raw materials. The present invention uses fermentable carbohydrates as raw materials, adds water-soluble surfactants, solid yeast powder, and fermentation inhibitors to the ethanol fermentation liquid, and carries out ultra-high-concentration ethanol fermentation without adding any nutrients; Inhibition of fermentation by toxic substances such as fatty acids, furfural, hydroxymethylfurfural, and levulinic acid. The present invention has the advantages that: the addition of water-soluble nonionic surfactants to the fermentation medium can effectively protect the survival rate of yeast cells from the influence of fermentation inhibitors, effectively improve the fermentation efficiency, and has a good application prospect .

Description

technical field [0001] The invention relates to the field of producing fuel ethanol through a fermentation process through the degradation of biomass raw materials, in particular to a method for improving yeast cell toxicity resistance fermentation performance by a surfactant. Background technique [0002] With the depletion of fossil energy and the aggravation of environmental pollution, the development and utilization of bioethanol, a renewable and clean energy, has attracted widespread attention. Lignocellulose is the most abundant biomass resource in the world, and the use of lignocellulose to produce fuel ethanol has great economic value and social significance. Pretreatment of lignocellulosic raw materials is an essential step in the production process of bioethanol. Dilute acid and steam explosion pretreatment are the pretreatment methods that have been studied more at present. In the process of dilute acid and steam explosion pretreatment, due to acid Under the acti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/10
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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