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Preparation method and application of a platinum-based alloy-labeled three-channel sandwich immunosensor

An immune sensor and platinum-based alloy technology, applied in the field of three-channel sandwich type immune sensor preparation, to achieve the effects of good conductivity, high catalytic performance, and strong electrical activity

Active Publication Date: 2015-12-02
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, three-channel immunosensors using non-precious metal platinum-based alloys, such as PtZn alloys, PtCd alloys, and PtCu alloys, as markers have not yet appeared.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker

[0030] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;

[0031] (2) Sonicate 3 μL, 1.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;

[0032] (3) Apply 3 μL and 8 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;

[0033] (4) Drop-coat 2 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...

Embodiment 2

[0036] Example 2 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker

[0037] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;

[0038] (2) Sonicate 4 μL, 2.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;

[0039] (3) Apply 4 μL and 10 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;

[0040] (4) Drop-coat 3 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...

Embodiment 3

[0043] Example 3 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker

[0044] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;

[0045](2) Sonicate 5 μL, 3.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;

[0046] (3) Apply 5 μL and 12 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;

[0047] (4) Drop-coat 4 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...

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PUM

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Abstract

The invention belongs to the technical field of nano functional material, immunoassay and biosensing and discloses a preparation method for platinum-base alloy-labeled three-channel sandwich type immunosensor and application. Through doping some non-precious metal alloy, the Pt load can be lowered, and accordingly the cost is reduced. By means of the good biocompatibility of Pt and the electro-activity of the other base metal, the Pt and base metal can serve as label to construct an electrochemical immunosensor, the simultaneous detection for three kinds of analyte is realized, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the technical fields of nanometer functional materials, immune analysis and biosensing, and in particular relates to a preparation method and application of a three-channel sandwich immunosensor with a platinum-based alloy as a marker. Background technique [0002] In recent years, the research on platinum-based alloy PtM has been greatly developed, especially as the best catalyst for low-temperature fuel cells, platinum-based alloy has attracted extensive interest of researchers. Compared with a single Pt nanomaterial, doping other metal alloys can reduce the loading of Pt, thereby reducing the cost, while keeping the catalyst activity unchanged or even improving the activity. In the construction of electrochemical sensors, platinum-based alloys are also commonly used, such as platinum, platinum-palladium, platinum-silver bimetallic alloys, or platinum-based trimetallic alloys. Alloys doped with other noble metals can improve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N27/327G01N27/48
CPCG01N27/3275G01N27/48G01N33/5438
Inventor 魏琴王晓东张森杜斌马洪敏庞雪辉罗川南曹伟吴丹范大伟
Owner UNIV OF JINAN
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