Preparation method and application of a platinum-based alloy-labeled three-channel sandwich immunosensor
An immune sensor and platinum-based alloy technology, applied in the field of three-channel sandwich type immune sensor preparation, to achieve the effects of good conductivity, high catalytic performance, and strong electrical activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0029] Example 1 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0030] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;
[0031] (2) Sonicate 3 μL, 1.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0032] (3) Apply 3 μL and 8 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;
[0033] (4) Drop-coat 2 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...
Embodiment 2
[0036] Example 2 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0037] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;
[0038] (2) Sonicate 4 μL, 2.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0039] (3) Apply 4 μL and 10 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;
[0040] (4) Drop-coat 3 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...
Embodiment 3
[0043] Example 3 Preparation method of a three-channel sandwich immunosensor with a platinum-based alloy as a marker
[0044] (1) Grind and polish the glassy carbon electrode with 0.1 μm and 0.05 μm alumina powder in turn, then rinse with ultrapure water to remove surface impurities, and complete the pretreatment of the glassy carbon working electrode;
[0045](2) Sonicate 5 μL, 3.0 mg / mL Pt-graphene aqueous solution to disperse evenly, then drop-coat it on the surface of the glassy carbon working electrode, and let it dry naturally;
[0046] (3) Apply 5 μL and 12 μg / mL of capture antibody for analyte a, capture antibody for analyte b, and capture antibody for analyte c to the surface of the working electrode respectively, and dry in a refrigerator at 4°C;
[0047] (4) Drop-coat 4 μL of bovine serum albumin with a mass fraction of 1% on the surface of the working electrode, place in a refrigerator at 4°C until dry, wash with ultrapure water, and dry in a refrigerator at 4°C; ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com