Sugar alcohol hydrophilic interaction chromatography stationary phase, synthesis and applications thereof

A chromatographic stationary phase and hydrophilic interaction technology, applied in the field of sugar alcohol hydrophilic interaction chromatographic stationary phase and its synthesis and application

Inactive Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of actual samples and the diversity of separation requirements, there is no hydrophilic interaction chromatography that can meet the separation requirements of most polar compounds. Therefore, it is very important to develop a new hydrophilic interaction chromatography stationary phase. Work

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sugar alcohol hydrophilic interaction chromatography stationary phase, synthesis and applications thereof
  • Sugar alcohol hydrophilic interaction chromatography stationary phase, synthesis and applications thereof
  • Sugar alcohol hydrophilic interaction chromatography stationary phase, synthesis and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention also relates to the preparation method of above-mentioned chromatographic stationary phase, and synthetic route is as follows Figure 6 Shown:

[0021] In the schematic diagram of the synthesis route of the sugar alcohol hydrophilic interaction chromatography stationary phase, ROH represents xylitol or maltitol.

[0022] The specific operation steps are as follows:

[0023] (1) Dissolve 5.0g of xylitol or maltitol in 100ml of anhydrous pyridine, and drop 6.8g or 3.0g of silane reagent (EtO) into the above solution through a dropping funnel at 80°C 3 SiCH 2 CH 2 CH 2 NCO, continue to react for 6h after the dropwise addition;

[0024] (2) Add 6.0 g of dry silica gel to the solution obtained from the reaction in (1), and reflux for 24 hours;

[0025] (3) After cooling, filter, and wash the solid product successively with pyridine, ethanol, water, ethanol and acetone, and then vacuum-dry at 60°C to obtain the target stationary phase.

[0026] Th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to synthesis of a sugar alcohol hydrophilic interaction chromatography stationary phase, and applications of the sugar alcohol hydrophilic interaction chromatography stationary phase in hydrophilic interaction chromatography. According to the present invention, xylitol or maltitol is adopted as a starting material, and a two-step reaction is performed to quickly and easily synthesize the target hydrophilic interaction chromatography stationary phase; and with application of the novel hydrophilic interaction chromatography stationary phase in the hydrophilic interaction chromatography, the good separation characteristic is provided in the separation of the polar organic compound and the water-soluble organic compound.

Description

technical field [0001] The invention relates to the design of the sugar alcohol hydrophilic interaction chromatography stationary phase, the invention also relates to the preparation method of the above stationary phase, and the application of the above stationary phase in the hydrophilic interaction chromatography. technical background [0002] In the traditional liquid chromatography separation mode, reversed-phase chromatography using C8 / C18 stationary phase is the most commonly used, it can meet the needs of most chromatographic separations, but reversed-phase chromatography is difficult to separate highly polar compounds, because such polarity Compounds are extremely weakly retained on C8 / C18 stationary phases and tend to elute at dead times. In order to solve the problem of liquid phase separation of such strong polar compounds, hydrophilic interaction chromatography came into being [Document 1: D.García-Gómez, E.Rodríguez-Gonzalo, R.Carabias-Martínez, Trends Anal.Chem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/286B01J20/30B01D15/08
Inventor 肖红斌用天万伯顺
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap