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THSD7A gene sequence, expression change detection, and application of sequence in coronary heart disease prediction

A technology of DNA sequence and coronary heart disease, which is applied in the detection of THSD7A gene sequence and expression changes and its application in the prediction of coronary heart disease, can solve the problems of lack of effective methods for susceptibility genes and insufficient explanation of the genetic basis of coronary heart disease, etc. To achieve the effect of reducing the incidence of

Active Publication Date: 2015-06-17
田小利 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, only 6 of the discovered loci are based on the research findings of the Chinese Han population, which is far from enough to explain the genetic basis of coronary heart disease in the Chinese Han population
In addition, most of the existing studies only focus on the related loci, and there is no effective method for identifying the susceptibility genes referred to by the related loci.

Method used

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  • THSD7A gene sequence, expression change detection, and application of sequence in coronary heart disease prediction
  • THSD7A gene sequence, expression change detection, and application of sequence in coronary heart disease prediction
  • THSD7A gene sequence, expression change detection, and application of sequence in coronary heart disease prediction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Genome-wide screening of coronary heart disease susceptibility loci:

[0070] Experimental population

[0071] In this embodiment, the patients with coronary heart disease (coronary angiography positive), that is, the samples and case data of the coronary heart disease case group came from the General Hospital of the Chinese People's Liberation Army, and the samples and data of the control group came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0072] Among them, the selection criteria for samples from the coronary heart disease case group meet one of the following three conditions:

[0073] (1) Previous medical history of ...

Embodiment 2

[0326] Example 2 Multi-center verification of rs17165136 locus

[0327] In order to further verify the rs17165136 single nucleotide polymorphism site in Example 1, this example will carry out multi-center verification on the above site. The method used for verification is still the combination of LCR and direct sequencing. The specific operation is as follows:

[0328] research population

[0329] The samples and data of patients with coronary heart disease (positive coronary angiography) used in the experiment came from the Affiliated Hospital of Harbin Medical University, China-Japan Friendship Hospital, Fuwai Hospital, and the First Affiliated Hospital of Zhengzhou University. The control samples and data came from the physical examination centers in Northeast and North regions that matched the regions of the cases; the case samples and data verified by the southern population came from the First People's Hospital of Jiangsu Province, the First People's Hospital of Ningbo...

Embodiment 3

[0342] Example 3 Identification of susceptibility genes

[0343] Unlike many SNP sites found in GWAS located in gene deserts, the rs17165136 site identified in this application is located in the THSD7A intron region, so the inventors hypothesized that the THSD7A gene may be a susceptibility gene for coronary heart disease. And verify it through this embodiment.

[0344] research population

[0345] The population used for eQTL analysis of THSD7A gene came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0346] cell culture

[0347] Human embryonic kidney cell line HEK293A was preserved by this experiment; primary human umbilical vein endotheli...

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Abstract

The invention relates to the biotechnical field, relates to a coronary heart disease susceptibility gene THSDA related single nucleotide polymorphism (SNP) site, a corresponding composition and a kit for detecting the SNP site, and an application of the nucleic acid affinity ligand of the SNP site in the preparation of compositions for detecting, screening and predicting the coronary heart disease susceptibility of Chinese Han population. The invention also relates to a corresponding composition and a kit for detecting the THSD7A gene, and an application of the nucleic acid affinity ligand of the THSD7A gene in the preparation of compositions for detecting, screening and predicting the coronary heart disease susceptibility of Chinese Han population. New further scientific bases are provided for the research and development of targeted coronary heart disease gene treatment, and a new clue is provided for founding of new coronary heart disease molecule mechanisms, development of new drug targets and the customized treatment of the coronary heart disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site on the THSD7A gene associated with susceptibility to coronary heart disease, and a corresponding combination for detecting the SNP site The invention relates to a substance and a kit, in particular to the application of the nucleic acid affinity ligand of the SNP site in the preparation of a composition for detecting, screening or predicting the susceptibility of coronary heart disease in Han population. Background technique [0002] Coronary heart disease, also known as coronary heart disease, is an ischemic heart disease caused by coronary artery wall atherosclerosis with morphological changes of coronary artery stenosis or even occlusion. Coronary heart disease is extremely harmful. According to the data of the World Health Organization, ischemic heart disease, mainly coronary heart disease, is the number ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 田小利李扬
Owner 田小利
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