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Method for preparing astragaloside IV by using Trichoderma viride

A technology of Trichoderma viride and astragaloside IV, applied in the field of biochemistry, can solve the problems of low yield of astragaloside IV, high cost of raw materials, complicated process and pollution, etc.

Active Publication Date: 2018-02-23
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of high raw material cost, low yield of astragaloside IV, and serious pollution of complex process, directly adopt the traditional Chinese medicine Astragalus as raw material, utilize Trichoderma viride to carry out microbial transformation, and then pass through macroporous adsorption resin, silica gel column chromatography and Purified by methanol recrystallization and other methods to finally obtain astragaloside IV with a purity of more than 98%

Method used

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  • Method for preparing astragaloside IV by using Trichoderma viride

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Embodiment 1

[0018] Traditional Chinese medicine Astragali is fermented by Trichoderma viride: After the commercially available freeze-dried powder of Trichoderma viride is activated, it is made into a Trichoderma viride test tube and stored on an inclined plane. Get a Trichoderma viride test tube and add 10ml sterile water to make spore suspension on the inclined surface, inoculate in (every bottle 1ml) in the 250ml shaking flask that 100ml liquid medium is housed, liquid medium is potato culture medium (200g potato adds water 1L, boil for 30-40min, filter through four layers of gauze to remove the filter residue, add 20g glucose to the filtrate (natural pH). Cultivate at 26° C. and 120 r / min for 48 hours to obtain seed liquid. Get 1ml seed liquid and join in 100ml fermentation medium, the formula of fermentation medium is 10g / L astragalus powder (the traditional Chinese medicine Radix Astragali is crushed and passed through 80 mesh sieve), 5g / L yeast powder, (NH 4 ) 2 SO 4 2.8g / L, KH...

Embodiment 2

[0021] The traditional Chinese medicine Astragalus is fermented by Trichoderma viride: add 10ml sterile water to the slant of Trichoderma viride test tube to make spore suspension, inoculate (1ml per bottle) in a 250ml shaking flask with 100ml liquid medium, and the liquid medium is potato medium (Add 1L of water to 200g of potatoes, boil for 30-40min, filter through four layers of gauze to remove the filter residue, add 20g of glucose to the filtrate, and adjust the natural pH). Cultivate at 26° C. and 120 r / min for 48 hours to obtain seed liquid. Get 10ml seed liquid and join in 1000ml fermentation medium, the formula of fermentation medium is 10g / L astragalus powder (the traditional Chinese medicine Radix Astragali is crushed and passed through 80 mesh sieve), 5g / L yeast powder, (NH 4 ) 2 SO 4 2.8g / L, KH 2 PO 4 4g / L, MgSO 4 ·7H 2 O 0.9g / L, anhydrous CaCl 2 0.9g / L, Tween 80 was added 1ml / L, and the pH was adjusted to 5.0. The fermentation broth was obtained by cult...

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Abstract

The invention relates to a method for preparing astragaloside IV by using Trichoderma viride. The method of the invention utilizes Trichoderma viride to carry out microbial transformation of the traditional Chinese medicine Astragalus membranaceus, and realizes the enrichment of the active ingredient astragaloside IV under certain fermentation and culture conditions. Then, the crude extract of astragaloside IV obtained by the biological method is separated and purified by macroporous adsorption resin HPD100, and further purified by silica gel column chromatography and methanol recrystallization to finally obtain astragaloside IV with a purity of more than 98%. The purpose of the method is to overcome the disadvantages of high raw material cost, low yield of astragaloside IV, complex process and serious pollution, etc., and is suitable for large-scale production of astragaloside IV with different purities.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to a new method for preparing astragaloside IV by transforming Trichoderma viride with the traditional Chinese medicine Astragalus as a raw material. Background technique [0002] Astragalus is sweet in nature and slightly warm, and is a commonly used traditional Chinese medicine for strengthening the body. The Chinese Pharmacopoeia stipulates that the traditional Chinese medicine Astragalus membranaceus is the dried root of Astragalus membranaceus (Fisch.) Bge and Mongolian Astragalus membranaceus (Fisch.) Bge.Var.mongholicus (Bge.) Hsiao. Its aliases are Mianqi and Mianhuangqi. Modern research has found that astragalus root contains a variety of useful ingredients. Among them, the root of Astragalus membranaceus contains astragaloside IV, isorhamnetin, quercetin, β-sitosterol, rhamnosin, linoleic acid and linolenic acid; Alkali, sucrose, amino acid, glucuronic acid and trace amount of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/20C12R1/885
Inventor 袁其朋王立媛
Owner BEIJING UNIV OF CHEM TECH