Feed additive and preparation method thereof
A feed additive and seed technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of difficult to achieve industrialization, standardization, non-compliance with basic functional principles, affecting the nutritional ratio of feed, etc., to enhance cellular immunity and body fluids. Effects of immunity, increased availability, increased daily gain and feed conversion ratio
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Embodiment 1
[0051] Preparation of Bacillus subtilis culture:
[0052] Obtain Bacillus subtilis fermentation broth by step-by-step expansion culture of slant strains;
[0053] (1) Primary seed cultivation: insert the slant strain of Bacillus subtilis into a 500-milliliter shake flask, with a medium loading capacity of 100 milliliters, a rotary shaker at 180 rpm, a cultivation temperature of 40° C., and a cultivation time of 12 hours;
[0054] (2) secondary seed cultivation: the primary seed is inserted into a 500 milliliter secondary seed shake flask according to the inoculum size of 10%, and the cultivation condition is the same as that of the primary seed;
[0055] (3) Tertiary seed cultivation: the secondary seeds are inserted into 5000 milliliters of tertiary seed shake flasks with 10% inoculum size, the medium loading capacity is 1000 milliliters, the rotary shaker is 100 rpm, and the cultivation temperature is 40 ° C. Time 12 hours;
[0056] (4) First-level seed tank cultivation: c...
Embodiment 2
[0063] A feed additive comprises the following raw materials in parts by weight: 8 parts of yeast cell wall extract, 30 parts of Bacillus subtilis culture, 9 parts of Astragalus extract; 7 parts of Bupleurum extract; score ratio. The preservation number of Bacillus subtilis Li-2013-02 is CGMCC No.7926.
Embodiment 3
[0065] The production method of the feed additive product described in Example 2: the yeast cell wall extract and fructooligosaccharide are crushed through a 60-mesh sieve, and the above crushed product is mixed with the culture of Bacillus subtilis, the extract of Astragalus membranaceus, and the extract of Bupleurum radix in proportion to obtain the feed additive .
[0066] Preparation of astragalus extract:
[0067] Crush the raw material astragalus until the particle size is less than 2 mm, add 5 times the weight of water to the container and mix evenly, control the temperature at 85°C, keep it for 3 hours, adjust the pH value to 6.5 with lactic acid, cool down to 52°C, add mixed enzymes, enzymes After decomposing for 3 hours, add a mixture of ethanol and propanol which is 2 times the weight of the mixed material, control the temperature to 75° C., keep it for 4 hours, and filter; the filtrate is concentrated in vacuum and then freeze-dried.
[0068] The added amount of t...
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