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Method for preparing antibacterial feed protein by utilizing aquatic product leftovers

A technology for aquatic product scraps and feed protein, applied in animal feed, animal feed, application and other directions, can solve problems such as increasing the amount of addition, improve utilization, good application prospects and social significance, improve immunity and disease resistance force effect

Inactive Publication Date: 2015-06-24
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the problem existing in the current solid-fermented antimicrobial peptide products, the purpose of the present invention is to prepare an antibacterial feed protein to significantly increase the amount of solid-fermented antimicrobial peptide products in the compound feed without affecting the design of the feed formula

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the Bacillus subtilis ( Bacillus subtilis ) HL-1 was inserted into the LB liquid medium cooled after sterilization, and cultured at 37 °C and 150 r / min for 16 hours to prepare Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution for use. The fresh tilapia leftovers are minced for later use; the soybean meal is crushed and passed through a 60-mesh sieve for later use. Mix 100 kg of minced tilapia scraps with 100 kg of crushed soybean meal passed through a 60-mesh sieve, add 400 g of glucose, 200 g of ferrous chloride, 500 g of dipotassium hydrogen phosphate and 10 kg of water and mix well , obtain the mixed fermentation medium; the mixed fermentation culture is based on 121 ℃ sterilization for 30min; then add 2 kilograms of Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution, stirred and mixed evenly; the fermented medium after inoculation was divided into shallow fermentation pans that had been surface-sterilized with 75% alcohol, and the th...

Embodiment 2

[0025] Embodiment 2: the Bacillus subtilis ( Bacillus subtilis ) HL-1 was inserted into the LB liquid medium cooled after sterilization, and cultured at 37 °C and 150 r / min for 16 hours to prepare Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution for use. Grind fresh shrimp heads for later use; crush peanut meal and pass through a 60-mesh sieve for later use. Mix 80 kilograms of minced shrimp heads with 100 kilograms of crushed peanut meal passed through a 60-mesh sieve, add 300 grams of glucose, 200 grams of ferrous chloride, 450 grams of dipotassium hydrogen phosphate and 10 kilograms of water to obtain Mixed fermentation medium; the mixed fermentation culture is based on 121°C sterilization for 30 minutes; then add 2 kg of Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution, stirred and mixed evenly; the raw materials after inoculation were divided into fermentation shallow plates that had been sterilized with 75% alcohol, the thickness of the material in ...

Embodiment 3

[0026] Embodiment 3: the Bacillus subtilis ( Bacillus subtilis ) HL-1 was inserted into the LB liquid medium cooled after sterilization, and cultured at 37 °C and 150 r / min for 16 hours to prepare Bacillus subtilis ( Bacillus subtilis) HL-1 seed solution for use. Grind the scallop skirt for later use; crush the cottonseed meal and pass it through a 60-mesh sieve for later use. 120 kilograms of minced scallop skirts are mixed with 100 kilograms of pulverized cottonseed meal passed through a 60-mesh sieve, and mixed with 250 grams of glucose, 150 grams of ferrous chloride, 400 grams of dipotassium hydrogen phosphate and 5 kilograms of water, Obtain a mixed fermentation medium; the mixed fermentation culture is sterilized at 121°C for 30 minutes; then add 2 kg of Bacillus subtilis ( Bacillus subtilis ) HL-1 seed solution, stirred and mixed evenly; the raw materials after inoculation were divided into fermentation pans that had been surface-sterilized with 75% alcohol. Steriliz...

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PUM

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Abstract

The invention discloses a method for preparing antibacterial protein by utilizing aquatic product leftovers. The method comprises the following main steps: mincing the fresh aquatic product leftovers for later use, crushing vegetable feed proteins, and filtering the crushed vegetable feed proteins by virtue of a 60-mesh screen for later use; mixing the minced aquatic product leftovers and the crushed vegetable proteins, and uniformly mixing nutrients and water to be used as a fermenting culture base; sterilizing and cooling the fermenting culture base, placing bacillus subtilis HL-1 capable of generating subtilin, and carrying out the solid aerobic fermentation; drying, crushing and packaging the fermented product to obtain the antibactieral feed protein. The prepared antibacterial feed protein is rich in subtilin, nutritional small peptide and probiotics and has a good function of promoting the growth and resisting the diseases. By adopting the antibacterial feed protein, not only can the utilization rate of the protein be increased, but also the antimicrobial-free feed can be prepared by utilizing the antibacterial feed protein, and the application prospect and the social benefit are good.

Description

technical field [0001] The invention relates to a method for preparing antibacterial feed protein by using leftovers of aquatic products, belonging to the field of processing and utilization of aquatic products. Background technique [0002] At present, 97,000 tons of the approximately 210,000 tons of antibiotic raw materials produced in China each year are used in animal husbandry, accounting for 46.1% of the total annual output. Excessive use of antibiotics not only leads to the resistance of farmed animals to antibiotics and reduces the disease resistance of farmed animals, but also these antibiotics will enter the human body along the food chain, causing the human body to inadvertently produce drug-resistant bacteria, which will increase once they get sick. Difficulty and cost of treatment, and even "super bacteria" may be produced, and there is no cure. With the continuous improvement of people's quality of life, there are higher requirements for the hygiene and safety...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A23K1/10A23K20/195
Inventor 洪鹏志刘唤明周春霞杨萍孙力军邓楚津
Owner GUANGDONG OCEAN UNIVERSITY
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