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Method for improving sulforaphene stability

A kind of technology of radhamone and stability, applied in the field of biochemical industry

Inactive Publication Date: 2015-06-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radish is still degraded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation and subpackage of PEG400 solution of SFE: Prepare PEG400 solution with a concentration of 90% SFE and place 2 mL in a 4 mL sample bottle.

[0023] Stability test of SFE in PEG400: 2 mL / bottle of 90% SFE-PEG400 solution was placed at 4°C and 26°C respectively, and samples were taken every week. And carry out high-performance liquid chromatography to detect the purity content detection of radhein. At 4 °C and 26 °C, the degradation rate of SFE was significantly reduced to 20.3% and 28.5% of the control group.

Embodiment 2

[0025] Preparation and subpackage of PEG600 solution of SFE: Prepare PEG600 solution with a concentration of 50% SFE and place 2mL in a 4mL sample bottle.

[0026] Stability test of SFE in PEG600: Place 2 mL / bottle of 50% SFE-PEG600 solution at 4°C and 26°C respectively, and take samples every week. And carry out high-performance liquid chromatography to detect the purity content detection of radhein. At 4 °C and 26 °C, the degradation rate of SFE was significantly reduced to 22.1% and 28.7% of the control group.

Embodiment 3

[0028] Prepare SFE soybean oil solution and subpackage: prepare 10% SFE-soybean oil solution, and take 2mL and place it in a 20mL sample bottle.

[0029] Stability test of SFE in soybean oil: Place 2 mL / bottle of 10% SFE-soybean oil solution at -20°C and 100°C respectively, and take samples every week. By water extraction (3 times*2mL) radhamin, the obtained radhamin aqueous solution was subjected to high performance liquid chromatography purity content detection. At -20 °C and 100 °C, the degradation rate of SFE was significantly reduced to 28.9% and 29.5% of the control group.

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Abstract

The invention relates to a method for improving sulforaphene stability. The sulforaphene is called SFE for short. The method comprises the following steps: dissolving the SFE into polyethylene glycol PEG, mixing uniformly, encapsulating in a closed container or dissolving the SFE in plant oil, fully mixing and encapsulating in the closed container. The added plant oil is one or more mixtures of soybean oil, corn oil, peanut oil, sesame oil, cottonseed oil, linseed oil, castor oil, rapeseed oil, sunflower seed oil, camellia oleosa seed oil and olive oil. By adopting the method, relative to the SFE, the water activity in the SFE is reduced, the stability of the SFE is improved, the stability of the SFE in the storage and transportation process is additionally achieved and the large-scale industrial application of the SFE for storage and transportation can be realized.

Description

Technical field: [0001] The invention belongs to the field of biochemical industry, and relates to two methods capable of significantly improving the stability of radishin. Background technique [0002] Cruciferous plants are now widely recognized as having anticancer properties. This is because cruciferous plants contain a lot of glucosinolates (glucosinolates). When plant cells are damaged, glucosinolates are hydrolyzed by myrosinase and rearranged intramolecularly to generate isothiocyanate, thiocyanate, nitrile and a small amount of cyclic thionitrile. Relevant studies have shown that isothiocyanate has good anticancer, antibacterial and antioxidant effects. [0003] Radish is an isothiocyanate compound extracted from radish seeds, radish sprouts, radish rhizomes and radish leaves. It can induce phase II detoxification enzyme activity and has good anticancer effect. [0004] However, our research has found that radishine is unstable to heat and will be degraded durin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/349A23L3/3472
Inventor 袁其朋田桂芳杨明邓炳华李媛程立刘翠婷王忠鹏刘玉婷
Owner BEIJING UNIV OF CHEM TECH
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