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Method for extracting white fungus polysaccharides at high temperature under high pressure

A tremella polysaccharide, high temperature and high pressure technology, applied in the field of polysaccharide extraction, can solve the problems of many impurities, low extraction rate, difficult to destroy the cell wall structure, etc., and achieve the effect of high purity, high extraction rate and simple extraction method

Inactive Publication Date: 2015-06-24
高枫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional methods for extracting tremella polysaccharides include: hot water extraction, enzymatic extraction, ultrasonic-assisted extraction, etc. Destroying the cell wall structure and fully releasing the fruiting body polysaccharide components, there are disadvantages such as low extraction rate and many impurities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for extracting tremella polysaccharides under high temperature and high pressure, comprising the following steps:

[0017] (1) select the white fungus without pests, put it in a pulverizer to pulverize, and after passing through a 40-mesh sieve, put it in a dryer and dry it for subsequent use;

[0018] (2) Add distilled water extractant to the container containing the dried white fungus powder at a material-to-liquid ratio of 1:60, seal with sulfuric acid paper, and place them in a water bath with a temperature of 100°C and a pressure of 0.2MP a Extracted in the autoclave for 2h;

[0019] (3) After the extraction time is over, the extract is squeezed with a filter cloth to filter out impurities, and placed in a centrifuge with a rotating speed of 4000r / min to centrifuge to obtain white fungus coarse sugar.

Embodiment 2

[0021] A method for extracting tremella polysaccharides under high temperature and high pressure, comprising the following steps:

[0022] (1) select the white fungus without pests, put it in a pulverizer to pulverize, and after passing through a 40-mesh sieve, put it in a dryer and dry it for subsequent use;

[0023] (2) Add distilled water extractant to the container containing the dried white fungus powder at a material-to-liquid ratio of 1:65, seal it with sulfuric acid paper, and place it in a water bath with a temperature of 105°C and a pressure of 0.3MP a Extracted in the autoclave for 2h;

[0024] (3) After the extraction time is over, the extract is squeezed with a filter cloth to filter out impurities, and placed in a centrifuge with a rotating speed of 4000r / min to centrifuge to obtain white fungus coarse sugar.

Embodiment 3

[0026] A method for extracting tremella polysaccharides under high temperature and high pressure, comprising the following steps:

[0027] (1) select the white fungus without pests, put it in a pulverizer to pulverize, and after passing through a 40-mesh sieve, put it in a dryer and dry it for subsequent use;

[0028] (2) Add distilled water extractant to the container containing the dried white fungus powder at a material-to-liquid ratio of 1:70, seal it with sulfuric acid paper, and place it in a water bath with a temperature of 110°C and a pressure of 0.4MP a Extracted in the autoclave for 2h;

[0029] (3) After the extraction time is over, the extract is squeezed with a filter cloth to filter out impurities, and placed in a centrifuge with a rotating speed of 4000r / min to centrifuge to obtain white fungus coarse sugar.

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Abstract

The invention provides a method for extracting white fungus polysaccharides at high temperature under high pressure, which comprises the following steps: (1) selecting insect-free white fungus, pulverizing in a pulverizer, passing through a 40-mesh screen, and drying in a drying machine; (2) adding a distilled water extractant into the container containing white fungus dry powder in a material / liquid ratio of 1:60-1:80, sealing with parchment paper, and extracting in a 100-120-DEG C water bath and a high-pressure sterilizer under the pressure of 0.2-0.6 MPa respectively for 2 hours; and (3) after finishing extraction, extruding the extracting solution through filter cloth to filter out impurities, and centrifuging in a centrifuge at the speed of 4000 r / min. The extraction method has the characteristics of high extraction yield, high purity, short time consumption and the like, is simple and easy to operate, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting tremella polysaccharides under high temperature and high pressure. Background technique [0002] Tremella, also known as Tremella, tremella, etc., belongs to the fungus Tremellaceae Tremella genus, is the fruiting body of Tremella, and has the laudatory title of "the crown of fungi". Tremella polysaccharide is an active polysaccharide extracted from Tremella fruiting bodies or spores. Modern structural analysis proves that Tremella polysaccharide is a heteropolysaccharide whose main chain is mannan composed of α-(1-3)-glycosidic bonds. , the 2, 4, and 6 positions of the main chain are connected with side chains composed of glucose, xylose, fucose and common uronic acid residues, and its active center is α-(1-3)-mannan. a common structural part. A large number of studies at home and abroad have proved that tremella polysaccharides have excellent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 高枫
Owner 高枫
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