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High-precision vibration slicing machine

A slicer, high-precision technology, applied in the field of research and medical equipment, can solve the problems affecting the slice quality, the Z-direction error of the vibrating slicer, etc., to avoid mechanical wear, reduce the Z-direction error, and reduce the effect of sample tissue damage

Active Publication Date: 2015-06-24
WUHAN OE BIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a high-precision vibrating slicer, aiming at overcoming the shortcomings of the vibrating slicer in the prior art that the error in the Z direction is serious and affects the slice quality

Method used

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Embodiment

[0031] The base is made of aluminum, and the spring steel sheet is made of carbon spring steel with an elastic modulus of 200kN mm -2, The external dimensions are 95×50×1.6mm, the vibration end is made of aluminum, and the natural vibration frequency of the single-degree-of-freedom vibration system is 80Hz. The coil is made of enamelled copper wire with a diameter of 0.35mm, a total of 1100 turns, and a resistance value of 32Ω. The coil bobbin is made of aluminum. The size of the permanent magnet is 20×30×10mm, and the residual magnetic strength is 1.4T. The driving force generated by the electromagnetic force driving structure is 46N / A. The fine-tuning screw adopts the fine-tuning screw with the specification of 170TPI produced by Newport Company, and the sensitivity is 0.4 μm. The elastic structure and the blade fixture are stainless steel processing parts. The blade is a zirconia blade from Electron Microscopy Sciences.

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Abstract

The invention relates to a high-precision vibration slicing machine. The machine comprises a base, a single-degree-of-freedom vibration structure, an electromagnetic driving structure, a blade adjusting rack and a blade, wherein the single-degree-of-freedom vibration structure comprises two thin-piece-shaped spring steel plates and a vibration end; one ends of the two thin-piece-shaped spring steel plates are respectively fixed to two ends of the base, while the other ends of the two thin-piece-shaped spring steel plates are connected through the vibration end, so as to achieve single-degree-of-freedom vibration; two permanent magnets of the electromagnetic driving structure are fixed on the vibration end; a coil framework of the electromagnetic driving structure is fixed on the base; an elastic structure body of the blade adjusting rack is fixed on the vibration end; the blade is fixed on the blade adjusting rack; the angle of the blade can be precisely adjusted through the blade adjusting rack. The machine is simple in structure; the Z-direction zero of the blade in vibration can be effectively controlled, so that the vibrating slicing precision can be improved, and the damage to the sample structure during slicing can be reduced.

Description

technical field [0001] The present invention relates to research and medical equipment, in particular to a high-precision vibrating microtome for slicing tissue samples. Background technique [0002] In modern biological research and medical examination, it is often necessary to use a microtome to make thin slices of biological tissue into certain thickness for further processing and observation such as staining and imaging. Vibrating microtome is a widely used type of slicer, which uses a certain device to drive the blade to reciprocate and vibrate along the blade direction (Y direction) to tear soft biological tissues, and at the same time the blade feeds along the X direction to realize the slicing process. Fresh living tissue or fixed tissue can be cut into thin sections ranging from tens of microns to hundreds of microns. In order to adapt to different biological tissues, parameters such as blade vibration amplitude, blade angle and sectioning speed are usually adjusta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/06
Inventor 龚辉骆清铭江涛袁菁
Owner WUHAN OE BIO CO LTD
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