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Portable type blood type system detection test paper and detection method thereof

A detection test strip, portable technology, applied in the field of portable blood group system detection test strips, can solve the problems of poor specificity of results, increase detection time, limit application value, etc., to eliminate false negatives and false positives, promote antigen-antibody reactions, and shorten blood type detection. effect of time

Active Publication Date: 2015-06-24
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the antigen and antibody at the quality control line need a second reaction, which increases the detection time
At the same time, because the color of the blood is red, the test line and quality control line are interpreted according to the red color, the error is large, and the specificity needs to be improved.
[0007] Noiphung et al. reported a blood type test strip that can detect ABO (A / B / AB / O) blood type and Rh (D) at the same time; The hematocrit (HCT) of the sample is lower than 42%, and it needs to be diluted 1:2 before testing, which is not easy to operate
At the same time, the general range of HCT in the normal population is about 37% to 50%, and the sample range of the detection is small, which cannot meet the blood type detection needs of ordinary people
Only relying on the distance ratio of red blood cells to plasma chromatography for result verification, lack of quality control controls, resulting in poor specificity of results
[0008] All the above-mentioned methods lack the quality control of the blood type detection device, there are certain false negatives and false positives, and the detection time is long, and it is impossible to truly realize POCT detection and meet the use in emergency situations, which greatly limits its application value

Method used

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  • Portable type blood type system detection test paper and detection method thereof
  • Portable type blood type system detection test paper and detection method thereof
  • Portable type blood type system detection test paper and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Preparation and use of positive and negative typing detection of human ABO blood type and Rh(D) blood type detection device

[0050] 1) Screening of solid-phase carrier: PVC is selected from (polyvinyl chloride material) PVC, polyethylene terephthalate (PET) and other materials as the solid-phase carrier bottom plate.

[0051] 2) Test paper matrix screening: Cotton pulp paper is selected from cotton pulp paper, printed paper, blotting paper, nitrocellulose membrane and other matrices as the test paper material for positive-setting sample pads, other swimming pads, and antibody pads.

[0052] 3) Anti-stereotype blood separation matrix screening: choose whole blood filter membrane as the matrix for separating RBC and plasma from materials such as whole blood filter membrane, nitrocellulose membrane, and cellulose acetate membrane.

[0053] 3) Reaction mat matrix screening: cotton pulp mat is selected as the reaction mat material from matrices such as cotton pul...

Embodiment 2

[0063] Example 2: Preparation and use of positive and negative typing detection of human ABO blood type and Rh(C) blood type detection device

[0064] Screening of solid phase carrier, test paper matrix and reaction pad, preparation and fixation of chromogenic reagent, preparation and dosage of washing solution, antibody titer and fixation method, detection conditions, etc. were carried out as described in Example 1.

[0065] 1) Detection card assembly: In the specific operation, batik and wax dipping techniques are preferably used according to the structural model of the accompanying drawing, and the channel is designed as any structure in the figure. In this embodiment, the Figure 4 shown as an example. Other methods such as microfluidic technology and chip technology can also be combined. According to detection requirements, in this embodiment, the Rh(D) channel is replaced by Rh(C) for detection, and any other antibody can also be replaced. Firstly, the rectangular cott...

Embodiment 3

[0069] Example 3: Preparation and use of positive and negative typing detection of human ABO blood type and Rh(E) blood type detection device

[0070] Screening of solid phase carrier, test paper matrix and reaction pad, preparation and fixation of chromogenic reagent, preparation and dosage of washing solution, detection conditions, etc. were carried out as described in Example 1.

[0071] 1) Detection card assembly: In the specific operation, batik and wax dipping techniques are preferably used according to the structural model of the accompanying drawing, and the channel is designed as any structure in the figure. In this embodiment, the Figure 5 shown as an example. Other methods such as microfluidic technology and chip technology can also be combined. According to detection requirements, in this embodiment, the Rh(D) channel is replaced by Rh(E) for detection, and any other antibody can also be replaced. First, the rectangular cotton pulp paper coated with 2-anti-A, 3-...

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Abstract

The invention discloses portable type blood type system detection test paper. The test paper is composed as follows: a solid-phase carrier is provided with a detection channel; a sample feeding cushion, an antibody cushion or an antigen cushion, a swimming cushion, a reaction cushion and a water absorption cushion are overlapped on the detection channel in sequence; the antibody cushion pre-coats a blood type detection agent; and a developing reagent is fixedly arranged on the reaction cushion. The test paper can be used for rapidly and accurately detecting a plurality of blood type systems including ABO, Rh, MNS, Kell, Kidd and the like, can be used for selecting items according to POCT detection requirements and can be suitable for various conditions including hospitals, families, wild emergency and the like.

Description

technical field [0001] The invention relates to the field of medical blood type detection, in particular to a portable blood type system detection test strip, which can quickly and accurately detect various blood type systems such as ABO, Rh, MNS, Kell, Kidd, etc., and can select items according to POCT detection needs, so as to be suitable for hospitals , family and field emergencies and many other situations. Background technique [0002] Blood type is a method of classifying blood, usually referring to the typing of red blood cells (RBC), which is based on whether there are some heritable antigenic substances on the surface of RBC. There are 32 blood group systems that have been discovered and recognized by the International Society of Blood Transfusion. The most closely related to human blood transfusion is the ABO blood group system, followed by the Rh blood group system, and MNS, Kell and other systems are also closely related to certain diseases. Blood typing refers ...

Claims

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Application Information

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IPC IPC(8): G01N33/80
CPCG01N33/54306G01N33/80
Inventor 罗阳张洪邱晓沛
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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