Fast creating method for rice long-grain japonica type indica-compatible two-line sterile line germplasm

A long-grain japonica and indica-friendly technology, applied in the field of crop genetics and breeding, can solve the problems of limiting the application potential of indica-japonica hybrid rice, low stigma exposure rate, high seed production cost, etc., to overcome application obstacles, healing rate and green seedling rate High and shorten the effect of breeding cycle

Active Publication Date: 2015-07-01
FUJIAN KINGSANTO DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the japonica male sterile lines currently in production are often due to factors such as late flowering time, low stigma exposure rate, and high seed production costs, which limit the application potential of indica-japonica hybrid rice in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) The long-grain indica rice maintainer line Jin 23B was crossed with the conventional japonica rice variety Xiushui 13 containing a wide-compatibility gene to obtain the first generation of hybrids. Plant the first generation of hybrids; when the rice grows and develops to the booting stage, after the young ears are retrieved, use M 8 Induction medium and Ms differentiation medium were used for anther culture to obtain anther culture plant H 0 About 1023 plants;

[0020] (2) Plant H 0 Plants, from which the long-grain indica type is selected, and the flowering time is early (the full flower is around 9:30 to 10:30 every morning), and the plant form is a naturally doubled diploid normal plant H of the japonica type. 1 210 plants;

[0021] (3) Using the above normal plant H 1 The plants were used as male parents, and II-32A and Gufeng A were respectively used as female parents for test crossing to obtain the corresponding first generation of hybrids;

[0022] (4)...

Embodiment 2

[0031] (1) The long-grain indica rice maintainer line Jin 23B was crossed with the conventional japonica rice variety Xiushui 13 containing a wide-compatibility gene to obtain the first generation of hybrids. Plant the first generation of hybrids; when the rice grows to the booting stage, take back the young ears, use M8 induction medium and Ms differentiation medium for anther culture, and obtain anther culture plant H 0 About 1023 plants;

[0032] (2) Plant H 0 Plants, from which the long-grain indica type is selected, and the flowering time is early (the full flower is around 9:30 to 10:30 every morning), and the plant form is a naturally doubled diploid normal plant H of the japonica type. 1 210 plants;

[0033] (3) Using the above normal plant H 1 The plants were used as male parents, and II-32A and Gufeng A were respectively used as female parents for test crossing to obtain the corresponding first generation of hybrids;

[0034] (4) Planting H in pairs 2 Generatio...

Embodiment 3

[0043] (1) The long-grain indica rice maintainer line Jin 23B was crossed with the conventional japonica rice variety Xiushui 13 containing a wide-compatibility gene to obtain the first generation of hybrids. Plant the first generation of hybrids; when the rice grows and develops to the booting stage, after the young ears are retrieved, use M 8 Induction medium and Ms differentiation medium were used for anther culture to obtain anther culture plant H 0 About 1023 plants;

[0044] (2) Plant H 0 Plants, from which the long-grain indica type is selected, the flowering habit is early (the full flower is around 9:30 to 10:30 every morning), and the plant form is a natural doubled diploid normal plant H of the japonica type 1 210 plants;

[0045] (3) Using the above normal plant H 1 The plants were used as male parents, and II-32A and Gufeng A were respectively used as female parents for test crossing to obtain the corresponding first generation of hybrids;

[0046] (4) Plant...

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PUM

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Abstract

The invention relates to a fast creating method for a rice long-grain japonica type indica-compatible two-line sterile line germplasm and belongs to the technical field of crop genetic breeding. The method relates to creation of the long-grain japonica type indica-compatible rice germplasm. By taking the two-line sterile line as a sterile gene donor, the rice two-line sterile line is bred by combining techniques of anther culture, natural condition and manual climatic box fertility identification and the like, wherein the plant shape is japonica type, the grain is the long-grain japonica, the flowering time is early, the fertility is stable and the comprehensive character is excellent. The sterile line is used for massive assembly of hybrid rice new combination, so that a first generation of strong heterotic indica/japonica hybrid is expected to be screened, thereby laying a foundation for effectively utilizing advantages of rice indica/japonica hybrid.

Description

technical field [0001] The invention specifically relates to a method for quickly creating germplasm of long-grain japonica-type parent-indica two-line sterile line of rice, and belongs to the technical field of crop genetics and breeding. Background technique [0002] The first generation of hybrid between indica and japonica subspecies of rice contains huge heterosis. For a long time, rice breeders have devoted themselves to the research on the improvement of rice yield by heterosis between indica and japonica subspecies. However, in breeding practice, it is difficult to breed strong dominant indica-japonica hybrid rice and apply it in a large area. The main problems are low seed setting rate of hybrids, too tall plants, long growth period and poor grain filling. For this reason, rice breeders have successively put forward the idea of ​​partially utilizing indica-japonica hybrid vigor, applying wide-compatibility genes and photosensitive genic sterile genes, indica-japoni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 练进旺万华雄林荔辉陈永发吴建梅林培清官智勤
Owner FUJIAN KINGSANTO DEV CO LTD
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