Cordycepin fermentation solid medium and preparation method and application thereof

A technology of solid culture medium and fermentation culture liquid, which is applied in the field of cordycepin fermentation solid medium and its preparation, can solve the problems of high production cost, difficult product purification, complicated synthesis process, etc. The effect of favoring accumulation

Active Publication Date: 2015-07-15
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high production cost of chemically synthesized cordycepin, complex synthesis process, low yield and difficult product purification, cordycepin is mainly prepared by biosynthesis

Method used

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  • Cordycepin fermentation solid medium and preparation method and application thereof
  • Cordycepin fermentation solid medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of solid medium for Cordyceps militaris fermentation.

[0030] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.

[0031] 2) Mix and stir the fermentation medium in step 1) and the expanded perlite with a particle size of 6mm according to the volume-to-mass ratio of 40ml / g. After spray drying, the water content of the material is controlled at 4ml / g and put into a fermenter at 121°C. Steam sterilize for 20 minutes to obtain a solid culture medium for cordycepin fermentation.

Embodiment 2

[0032] Example 2: Preparation of Cordyceps militaris fermentation medium.

[0033] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.

[0034] 2) Mix and stir the fermentation medium in step 1) and the expanded perlite with a particle size of 4mm according to the volume-to-mass ratio of 30ml / g. After spray drying, the water content of the material is controlled at 5ml / g and put into a fermenter at 121°C. Steam sterilize for 20 minutes to obtain a solid culture medium for cordycepin fermentation.

Embodiment 3

[0035] Example 3: Preparation of Cordyceps militaris fermentation medium.

[0036] 1) Glucose 42g / L, yeast extract 6g / L, peptone 10g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.5g / L, Tween 80 2g / L, The solvent is water, and the pH value of the solution is adjusted to 5.8 after fully stirring and dissolving to obtain a fermentation culture solution.

[0037] 2) The fermentation culture liquid in step 1) is mixed with the expanded perlite with a particle size of 8mm according to the volume to mass ratio of 50ml / g. After spray drying, the water content of the material is controlled at 2.5ml / g and loaded into a fermenter. 121 ℃ steam sterilization for 20 minutes to obtain a solid medium for cordycepin fermentation.

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Abstract

The invention discloses a cordycepin fermentation solid medium and a preparation method and application thereof. The medium is prepared through the following steps of firstly, fully stirring and dissolving glucose, yeast extract, peptone, monopotassium phosphate, dipotassium phosphate, magnesium sulfate and Tween 80 with water as solvent, and adjusting the pH value of the solution to be within the range of 5.5 to 6.0 to prepare a fermentation culture solution; secondly, evenly mixing the fermentation culture solution obtained in the first step with expanded perlite, filling a fermentation tank with the mixture after spray drying is conducted on the mixture, sterilizing the mixture through vapor under the temperature of 121 DEG C for 20 minutes, and obtaining the cordycepin fermentation solid medium. The cordycepin fermentation solid medium is large in sporocarp amount, high in synchronism and strong in viability; the growth of cordyceps militaris and the accumulation of cordycepin are promoted by compensating for cordycepin precursor adenine and glycerol trioleate through spraying, and the yield and quality of cordycepin can be improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a solid medium for cordycepin fermentation and its preparation method and application. Background technique [0002] Cordycepin is one of the main active ingredients in Cordyceps. Cordycepin has a variety of biological activities, such as anti-tumor, anti-proliferation, anti-metastasis, antibacterial, antiviral, immune regulation, and anti-inflammation. The preparation of cordycepin mainly includes chemical synthesis and biosynthesis. Due to the high production cost of chemically synthesized cordycepin, complex synthesis process, low yield and difficult product purification, cordycepin is mainly prepared by biosynthesis. There are two ways to prepare cordycepin by biosynthesis: one is to obtain Cordyceps fruiting body by solid fermentation, and then extract it; the other is to directly extract from the fermentation broth through liquid fermentation of Cordyce...

Claims

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Application Information

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IPC IPC(8): C12P19/40C12R1/645
Inventor 汤佳鹏
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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