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Double-dyeing kit for cervical intraepithelial neoplasia grading assisted diagnosis, and applications thereof

A technology for auxiliary diagnosis and kits, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of affecting treatment plan selection and prognosis, interfering with CIN grading, low specificity, etc., to increase readability and accuracy, The effect of accurate CIN classification and accurate information

Inactive Publication Date: 2015-07-22
GUANGZHOU LBP MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In oncogenic human papillomavirus (HPV)-related squamous intraepithelial neoplasia (SILs), the sensitivity of p16 marker detection reaches 89%-98%, but because oncogenic HPV widely appears in all levels of SILs , so the specificity of p16 is not high, for example, p16 is also positive in a small part of negative and reactive cervical biopsy epithelial tissue, which interferes with the classification of CIN and affects the choice of treatment options and prognosis

Method used

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  • Double-dyeing kit for cervical intraepithelial neoplasia grading assisted diagnosis, and applications thereof
  • Double-dyeing kit for cervical intraepithelial neoplasia grading assisted diagnosis, and applications thereof
  • Double-dyeing kit for cervical intraepithelial neoplasia grading assisted diagnosis, and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0059] The research object was formalin-fixed-paraffin-embedded normal cervical epithelial tissue (provided by Guangzhou Da'an Clinical Laboratory Center Co., Ltd.), and the grading and identification steps were as follows:

[0060] 1) Dewaxing and hydration: Before dewaxing, place tissue slices on a 60°C oven for 2 hours, soak in xylene for 10 minutes x 2 times, soak in absolute ethanol for 5 minutes x 2 times, 95% ethanol Soak in water for 5 minutes, soak in distilled water for 1 minute;

[0061] 2) Antigen retrieval: slices were repaired by boiling in EDTA antigen retrieval solution with a pH of 9.0, cooled, rinsed with tap water, and soaked in distilled water;

[0062] 3) Sealing: incubate with 3% hydrogen peroxide at room temperature for 10 minutes, rinse with distilled water, wash with TBS and soak;

[0063] 4) Double staining: add Stathmin antibody and MCM2 antibody dropwise, the titers of both antibodies are 100; incubate at room temperature for 60 minutes, wash with ...

Embodiment 2

[0070] The research objects were formalin-fixed-paraffin-embedded CINI and CINII cervical lesion tissues (provided by Guangzhou Da'an Clinical Laboratory Center Co., Ltd.), and the grading and identification steps were as follows:

[0071] 1) Dewaxing and hydration: Before dewaxing, place tissue slices on a 60°C oven for 2 hours, soak in xylene for 10 minutes x 2 times, soak in absolute ethanol for 5 minutes x 2 times, 95% ethanol Soak in water for 5 minutes, soak in distilled water for 1 minute;

[0072] 2) Antigen retrieval: slices were repaired by boiling in EDTA antigen retrieval solution with pH 9.0, cooled, rinsed with tap water, and soaked in distilled water;

[0073] 3) Sealing: incubate with 3% hydrogen peroxide at room temperature for 10 minutes, rinse with distilled water, wash with TBS and soak;

[0074] 4) Double staining: add Stathmin antibody and MCM2 antibody dropwise, the titers of both antibodies are 100; incubate at room temperature for 60 minutes, wash wit...

Embodiment 3

[0081] The research object was formalin-fixed-paraffin-embedded CINIII cervical lesion tissue (provided by Guangzhou Da'an Clinical Laboratory Center Co., Ltd.), and the grading and identification steps were as follows:

[0082] 1) Dewaxing and hydration: Before dewaxing, place tissue sections on a 60°C oven for 2 hours, soak in xylene for 10 minutes x 2 times, soak in absolute ethanol for 5 minutes x 2 times, and soak in 95% ethanol Soak for 5 minutes, soak in distilled water for 1 minute;

[0083] 2) Antigen retrieval: slices were repaired by boiling in EDTA antigen retrieval solution with pH 9.0, cooled, rinsed with tap water, and soaked in distilled water;

[0084] 3) Sealing: incubate with 3% hydrogen peroxide at room temperature for 10 minutes, rinse with distilled water, wash with TBS and soak;

[0085] 4) Double staining: add Stathmin antibody and MCM2 antibody dropwise, the titers of both antibodies are 100; incubate at room temperature for 60 minutes, wash with TBS,...

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Abstract

The present invention discloses a double-dyeing kit for cervical intraepithelial neoplasia grading assisted diagnosis, and applications thereof, wherein the double-dyeing kit comprises an immunohistochemisty marker first antibody, and the immunohistochemisty marker first antibody is a mixed antibody reagent comprising Stathmin antibody and MCM2 antibody. With the application of the double-dyeing kit in the cervical intraepithelial neoplasia grading assisted diagnosis, the two immunohistochemisty markers are adopted as the mixed antibody so as to increase the sensitivity and the specificity of the CIN grading and accurately identifying the CIN grading; and the readability and the accuracy of the film reading are increased, the detection cost is reduced, and the operation steps are reduced.

Description

technical field [0001] The invention belongs to the field of immunohistochemistry, and in particular relates to a double-staining kit for grading and auxiliary diagnosis of cervical intraepithelial neoplasia and its application. Background technique [0002] Cervical cancer is a common female malignant tumor, and it is the only known preventable cancer. Internationally, cervical cancer preventive vaccines have been launched on the market. If it is detected before or at an early stage, cervical cancer has a high cure rate. The occurrence and development of cervical cancer is a long gradual process. There is a long and reversible precancerous lesion period. It takes about ten years to develop from cervical intraepithelial neoplasia (CIN) to cancer. The cervix is ​​easy to directly observe and obtain materials. As long as precancerous lesions can be detected and treated correctly, cervical cancer can be prevented. [0003] Timely detection and correct treatment of cervical pre...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57411
Inventor 杨鉴李玉闫有臣张海明
Owner GUANGZHOU LBP MEDICINE SCI & TECH
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