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Tissue culture and rapid propagation method for radix cynanchi bungei

A technology of rapid propagation of Radix Polygoni Multiflori, applied in the field of plant tissue culture, can solve the problems of unsatisfactory wild resources, decline in yield and quality, and exhaustion of wild resources, so as to speed up breeding and promotion, reduce production costs, and improve reproductive efficiency Effect

Inactive Publication Date: 2015-07-29
冯文杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of production and the continuous improvement of living standards, people's demand for medical and health care products is increasing day by day, wild resources can not meet the needs, and will soon lead to the depletion of wild resources, so it is necessary to carry out artificial cultivation
At present, the artificial cultivation of Baishouwu mainly depends on the vegetative propagation of root tubers and seedlings, which is susceptible to virus infection, leading to the degradation of species, the decline of yield and quality, and cannot meet the needs of large-scale and standardized production. Therefore, it is necessary to establish a tissue culture A complete set of techniques for rapid propagation of Baishouwu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation and disinfection of explants: Select mature and plump Baishouwu seeds, soak them in clean water for 24 hours, disinfect them with 75% ethanol for 30 seconds on an ultra-clean workbench, and then disinfect them with 0.1% mercury chloride for 10 minutes. Then rinse with sterile water 3 times, dry the water with sterile filter paper, and inoculate into 1 / 2 MS medium (0.4% agar, 3.0% white sugar, pH5.8). Afterwards, they were placed in light for 12 hours every day, and the light intensity was 1000 lx and cultivated for 14 days.

[0018] (2) Induction culture: Cut the stem section with axillary buds of the aseptic seedlings obtained in step (1) into about 0.5 cm, put the top end upwards on the induction medium, and keep the axillary bud points at the nodes on the surface of the medium. After inoculation, it was placed in the light for 12 hours a day, the light intensity was 1000lx, the culture temperature was 25°C, and the relative air humidity was 75% f...

Embodiment 2

[0023] (1) Preparation and disinfection of explants: Select mature and plump Baishouwu seeds, soak them in clean water for 48 hours, disinfect them with 75% ethanol for 40 seconds on an ultra-clean workbench, and then disinfect them with 0.1% mercury chloride for 15 minutes. Then rinse with sterile water 5 times, dry the water with sterile filter paper, and inoculate into 1 / 2 MS medium (0.4% agar, 3.0% white sugar, pH5.8). Afterwards, they were placed in light for 12 hours every day, and the light intensity was 1000 lx and cultivated for 25 days.

[0024] (2) Induction culture: Cut the stem section with axillary buds of the aseptic seedlings obtained in step (1) into about 0.5 cm, put the top end upwards on the induction medium, and keep the axillary bud points at the nodes on the surface of the medium. After inoculation, it was placed in the light for 13 hours a day, the light intensity was 1500lx, the culture temperature was 26°C, and the relative air humidity was 75% ...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method for radix cynanchi bungei. Radix cynanchi bungei is an asclepiadaceous cynanchum plant, has tuberous root used as a medicine and is sweet in taste, bitter and slightly warm in nature. Main effective ingredients of the radix cynanchi bungei are C21 steroidal glycosides, so that the radix cynanchi bungei has effects of replenishing blood and vital energy, resisting ageing, reducing blood fat and cholesterol and promoting growth of dark hairs and is nontoxic. A radix cynanchi bungei seed is taken as an explant, an in-vitro regeneration plant of the radix cynanchi bungei is obtained through processes of explant disinfection, adventitious bud induction, multiplication culture, rooting culture, acclimatization and transplantation and the like, and an effective tissue culture and rapid propagation technical system of the radix cynanchi bungei is created, so that a large amount of seeds and seedlings can be produced within a short time, the propagation efficiency is improved, the production cost is reduced, and the propagation and popularization of the radix cynanchi bungei are accelerated.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Radix Polygoni Multiflori. Background technique [0002] Baishouwu is a plant of the genus Evelyn in the family Rumoaceae. Its root tubers are used as medicine, with sweet, bitter and slightly warm nature. The main active ingredient of Baishouwu is C 21 Steroidal glycosides are also rich in starch, protein, vitamins and minerals. Baishouwu has the effects of nourishing blood, nourishing qi, anti-aging, lowering blood fat, lowering cholesterol and promoting the growth of Aconitum hair, and it is non-toxic. In production, Baishouwu is used to produce high-quality starch, which has high economic value. [0003] Generally, Baishouwu comes from wild resources. With the development of production and the continuous improvement of living standards, people's demand for medical and health care pro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯文杰
Owner 冯文杰
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