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Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method

A technology of subcutaneous tissue and tissue cage, which is applied in the fields of application, medical science, ovulation diagnosis, etc. It can solve the problems of being unsuitable for Beagle dogs and large size, and achieves the effects of convenient acquisition, simple production process, and low price

Inactive Publication Date: 2015-07-29
苏州圣苏新药开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this model is bulky and not suitable for beagle dogs with tighter skin

Method used

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  • Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method
  • Tissue cage model for collecting subcutaneous tissue fluid of beagle and using method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The main body of the tissue cage model is made of medical silicone tube (1), which has good biocompatibility. The specifications of the tissue cage model are: outer diameter 18.0 mm, inner diameter 13.0 mm, length about 65±2 mm, volume about 8.0 cm 3 ;Punch 8 small holes with a diameter of 2.0 mm at both ends of the medical silicone tube (2), so that the tissue cage and the external liquid exchange area is 50.3 mm 2 . The medical silicone disc (4) is first sutured to the two ends of the medical silicone tube (2) with nylon thread, and then 705 silicone glue is used to seal the gap.

[0023] After the finished tissue cages were cleaned, they were soaked and disinfected with bromogeramine solution overnight, and then dried for later use. Beagle dogs were first given general anesthesia with pentobarbital sodium, the backs of both sides were shaved, iodine tincture was disinfected and alcohol deiodized, the operation was performed local anesthesia with procaine hydrochl...

Embodiment 2

[0025] Determine the best silicone tube specifications through controlled experiments:

[0026] Select medical silicone tubes of different specifications, make a tissue cage model according to Example 1, and transplant it into the subcutaneous tissue of the dog's back, observe continuously for 1-2 months, and collect tissue fluid to evaluate the success rate of the tissue cage model, so as to select the tissue model for making Optimal size of medical silicone tubing. The results are as follows:

[0027]

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Abstract

The invention relates to a tissue cage model for collecting the subcutaneous tissue fluid of beagle and a using method. A medical silicone tube is adopted as a main body of the tissue cage model, wherein at least four uniformly distributed small tissue fluid exchange holes with diameters of 2mm are formed in each of the two ends of the medical silicone tube; two end openings of the medical silicone tube are sealed by medical silicone round sheets; the tissue cage model has an external diameter of 16.0 to 20.0mm, an internal diameter of 11.0 to 15.0mm, a length of 63 to 67mm and a volume of about 6.0 to 11.0cm<3>. After being shaped, cleaned and sterilized, the model is implanted into the subcutaneous tissue layer on the two sides of the back of the beagle in a surgical operation manner, and after 4 to 5 weeks, clear yellowish fluid in a tissue cage of an uninfected wound is tissue fluid. A disposable injector externally pierces the tissue cage to extract the tissue fluid for researches in related directions of in-vivo tissue fluid pharmacokinetics and pharmacodynamics of drugs, in-vivo antibacterial activity of antibiotics, drug resistance and the like.

Description

technical field [0001] The invention relates to the field of drug test research, in particular to a tissue cage model for collecting animal subcutaneous tissue fluid. Background technique [0002] Drugs that enter the body through different administration methods first reach the blood through absorption, and then are transported to the target organs and tissues of the body. Traditional pharmacokinetic studies measure drug concentrations in plasma or serum. However, there are different osmotic ratios in plasma and organ tissues, and the drug concentration in plasma or serum cannot fully represent the actual drug concentration in tissues. Another large body fluid in the body, interstitial fluid, is the necessary place for pathogenic microorganisms or their toxins to damage parenchymal cells. It is also the place where various drugs treat damaged tissues or cells and exert their medicinal effects. Therefore, when we conduct pharmacological research on drugs, the pharmacokinet...

Claims

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Application Information

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IPC IPC(8): A61B10/00
Inventor 张炳旭郭建军卜海之
Owner 苏州圣苏新药开发有限公司