Composite moringa polypeptide and preparation method and application thereof
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A technology of Moringa oleifera and protease, which is applied in the field of compound Moringa oleifera polypeptide and its preparation, can solve problems such as no records, and achieve the effects of alleviating physical fatigue, high biological activity, and enhanced activity
Active Publication Date: 2015-08-12
ZHANJIANG TONGLING MEDICAL & BIOLOGICAL ENG CO LTD
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At present, there are no records on the related research of using Moringa as raw material to obtain small mol
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Embodiment 1
[0031] The preparation of embodiment one compound Moringa polypeptide
[0032] 1) Pre-treatment of raw materials: Take Moringa oleifera roots, stems, leaves, and flowers in a weight ratio of 1:5:6:1, screen, wash, dry at a constant temperature of 50-60°C, and crush them into particles below 10 mesh.
[0033] 2) Enzymolysis reaction: put the pretreatment raw materials into the enzymolysis tank, add 5-10 times the weight of pure water, heat to 40-60°C, stir, adjust the pH value to 5.5-6.5, according to Moringa: enzyme Add protease at a weight ratio of 100:0.1 to 1, enzymolyze for 4 to 6 hours under stirring, and separate the supernatant; the protease is one or more selected from subtilisin, chymotrypsin and phosphodiesterase .
[0034] 3) Second enzymatic hydrolysis: Add 3 to 5 times the remaining residue to 40-60°C distilled water for 2 enzymatic hydrolysis, and take the supernatant, the method is the same as the enzymatic hydrolysis method in the previous step;
[0040] In parts by weight, add 50-80 parts of compound Moringa polypeptide granules, add 5-20 parts of microcrystalline cellulose, 1-3 parts of magnesium stearate, mix well, press into tablets, 0.3g / piece, packing: 30 pieces / piece bottle. Dosage: Take it in the morning and evening, 3 tablets each time. Embodiment 3 Compound Moringa Polypeptide Capsules
Embodiment 3
[0041] In parts by weight, 50-80 parts of compound Moringa polypeptide powder, 5-20 parts of dextrin and 1-2 parts of magnesium stearate are added, mixed well, filled with capsules, 0.3g / capsule, packed in aluminum foil, 10 capsules / Version. Usage and dosage: take 3 capsules each time, once in the morning and at night.
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Abstract
The invention discloses composite moringa polypeptide and a preparation method and application thereof, moringa polypeptide is extracted from a moringa raw material by enzymatic hydrolysis method, and then is added with salvia miltiorrhiza, ginseng, Chinese wolfberry extract and other traditional Chinese medicines for preparation of a compound preparation, the moringa polypeptide is further used in medicine, food and other fields for full application of the active ingredients in moringa. Micro-molecule polypeptides is obtained by low temperature enzymolysis of moringa protein under the effect of controlled protease by biological engineering technology method, the molecular weight of the micro-molecule polypeptides is 1-10 KD, the obtained composite moringa peptide raw material has the effects of oxidation resistance, protection of heart and brain cells against super oxygen free radical damage, prevention and treatment of cardiovascular and cerebrovascular diseases, relieving of fatigues, and regulation of body's metabolism balance, not only can be used in drugs, can be made into pharmaceutical preparations (tablets, capsules, granules, oral liquid and other dosage forms), and also can be used in health food, food and cosmetics.
Description
technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a compound Moringa oleifera polypeptide and its preparation method and application. Background technique [0002] Moringa is a plant of the genus Moringa in the Moringaceae family, which originated in the southern foothills of the Himalayas in northwestern India. Studies have found that in addition to rich nutrition, Moringa is also used to treat diabetes, high blood pressure, skin diseases, low immunity, anemia, bone diseases, anti-depression, arthritis, digestive organ tumors and other diseases. As a functional plant, Moringa has broad development prospects. The whole plant of Moringa oleifera can be used, and there are many types of nutrients. The crude protein content of Moringa oleifera is 23.0% to 43.5%, and it is rich in V A , V B , V C , V E And calcium, potassium, iron and other mineral nutrients, in addition, it also contains various amino a...
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