Double room hypha test apparatus and double room hypha test method

A test device and test method technology, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the problems that the influence cannot be eliminated, the mass flow diffusion cannot be effectively controlled, and the mass flow diffusion can be reduced. The effect of good isolation, the effect of broadening the scope of research

Inactive Publication Date: 2015-08-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there are also disadvantages: the influence of mass flow diffusion between the mycelial chamber and the plant chamber of highly mobile elements in the soil cannot be ruled out
[0004] The traditional compartmental device is arranged left and right. In this case, the plants need to form a root pad on the side, and then the mycelium reaches the mycelium chamber from the side, which cannot effectively control the influence of mass flow diffusion.

Method used

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  • Double room hypha test apparatus and double room hypha test method
  • Double room hypha test apparatus and double room hypha test method
  • Double room hypha test apparatus and double room hypha test method

Examples

Experimental program
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Embodiment 1

[0029] Such as figure 1 , Figure 4 and Figure 5 It can be seen that a two-chamber mycelium test device includes from top to bottom: a plant chamber 1, a filter screen 13, an air barrier 14, and a mycelium chamber 2; the plant chamber 1 is cylindrical with an opening at the top and closed sides , the bottom is provided with a filter screen 13 and an air compartment 14; the mycelium chamber 2 is the lower device of the test device, and the mycelium chamber 2 is a cylindrical structure with an upper opening, and the side is provided with a ventilation hole 25 , the bottom is a fully enclosed structure; the plant chamber 1 and the mycelium chamber 2 are fixed by bolts, and can be detachably combined.

[0030] Such as Figure 6 It can be seen that the filter screen 13 is on the air interlayer, the grid size is uniform, and the grid is evenly arranged; the air interlayer 14 is a rigid plastic plate with round holes; further, the rigid plastic plate is a PVC plate , polycarbona...

Embodiment 2

[0034] A two-chamber mycelium test method is as follows: step 1: plants are planted in the plant room 1; step 2: the cultivation soil and straw are placed in the mycelium chamber 2, the straw is placed on the cultivation soil, Put earthworms or other soil animals in the mycelium chamber 2 as required; Step 3: place the plant chamber 1 above the mycelium chamber 2, and fix the whole device with bolts; Step 4: Conduct experimental observation.

[0035] Plants are cultivated and studied in the installed device. Most of the plant rhizomes are in the plant room 1, wherein the mycelium enters the mycelium room 2 through the gap between the filter screen 13 and the air barrier 14, and is covered in the mycelium room 2. There are soil for cultivation, straw and fertilizer, etc., and soil animals for research are added in the mycelium room 2. By putting in different soil animals, the influence and effect of various soil animals on the mycelium can be studied.

[0036] The two-chamber m...

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Abstract

The present invention relates to the field of arbuscular mycorrhiza fungi test apparatus, particularly to a double room hypha test apparatus and a double room hypha test method. The double room hypha test apparatus comprises a plant room, a filtration screen, an air separation layer and a hypha room from top to bottom, wherein the upper portion of the plant room is provided with an opening, the edge side of the plant room is sealed, the filtration screen and the air separation layer are arranged on the bottom portion of the plant room, the upper portion of the hypha room is provided with an opening, the edge side of the hypha room is provided with a ventilation gas permeation hole, the bottom portion of the hypha room is the completely-closed structure, and the plant room and the hypha room are connected through a bolt. According to the present invention, with the air separation layer method, the root system and the hypha can be well separated; and the upper compartment and the lower compartment are adopted, such that the gravity effect can be well utilized, the hypha growth is easily achieved, the nutrient diffusion influence between the two rooms due to the mass flow can be reduced, and the good test effect is achieved.

Description

technical field [0001] The invention relates to the field of arbuscular mycorrhizal fungus test devices, in particular to a two-chamber mycelium test device and method. Background technique [0002] Arbuscular mycorrhiza (AM) fungi are the most widely distributed mycorrhizal types, and 80% to 90% of terrestrial angiosperms can form arbuscular mycorrhiza. The scope for plants to absorb nutrients from the soil is limited. After the AM fungus infects plants, the extra-root hyphae can extend widely through the rhizosphere nutrient deficient zone into the soil, forming a mycelium, and expanding the scope of root nutrient absorption. [0003] Under natural conditions, AM fungi and root symbiosis are associated with each other, and it is difficult to distinguish the influence of roots or hyphae on soil nutrient uptake. Li Xiaolin (1990) used a three-chamber test device to separate the roots from the hyphae using a 30 μm nylon mesh to establish the mycelium. This method provides a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12R1/645
CPCC12N1/14
Inventor 李欢
Owner QINGDAO AGRI UNIV
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