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Detection method of sulfite in food

A sulfite and detection method technology, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials, to achieve the effects of high detection accuracy, reliable detection results and low price

Inactive Publication Date: 2015-08-12
WUZHOU INST OF PROD QUALITY INSPECTION
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In addition, because sulfur contains lead, arsenic, thallium, etc., these toxic substances can enter the food during fumigation

Method used

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  • Detection method of sulfite in food
  • Detection method of sulfite in food
  • Detection method of sulfite in food

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Experimental program
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Embodiment 1

[0025] 1. Reagents

[0026] 1. Sodium tetrachloromercury absorption solution: Weigh 13.6g of mercuric chloride and 6.0g of sodium chloride, dissolve in water and dilute to 1000mL, leave overnight, filter and set aside.

[0027] 2. Ammonium sulfamate solution (12g / L),

[0028] 3 Formaldehyde solution (2g / L): absorb 0.55mL of formaldehyde (36%) without polymerization precipitation, add water to dilute to 100mL, and mix well.

[0029] 4. Starch indicator solution: Weigh 1g of soluble starch, make a paste with a little water, slowly pour it into 100ml of boiling water, stir as it is added, boil, and let it cool for later use. This solution is prepared immediately before use.

[0030] 5. Potassium ferrocyanide solution: weigh 10.6g potassium ferrocyanide (K 4 Fe(CN) 6 3H2O), add water to dissolve and dilute to 100ml,

[0031] 6. Zinc acetate solution: weigh 22g of zinc acetate [Zn(CH 3 000)2·2H 2 0] Dissolve in a small amount of water, add 3ml of glacial acetic acid, dilute t...

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Abstract

The invention discloses a detection method of sulfite in food. The invention aims to provide the detection method which has advantages as follows: materials are cheap and easily available; and detection time is short. Technical points of the detection method comprise the following steps: 1, sample processing; 2, determination: absorbing 0.50-5.0 ml of a sample treating fluid into a 25ml corked colorimetric tube, absorbing 0.20, 0.40, 0.60, 0.80, 1.00, 1.50 and 2.00 ml of sulfur dioxide standard working solutions and respectively putting the sulfur dioxide standard working solutions into the 25ml corked colorimetric tube; respectively adding sodium tetrachloromercurate absorption liquids to 10 ml into the sample and a standard pipe, adding 1 ml of an iron sulfamate solution, 1 ml of a formaldehyde solution and 1 ml of basic fuchsin, uniformly shaking, standing for 20 min, adjusting zero point with a zero tube by using a 1cm colorimetric cup, measuring absorbancy at the wavelength of 550 nm, drawing a standard curve and comparing; and 3, calculation. The invention belongs to the field of a food detection technology.

Description

technical field [0001] The invention relates to a method for detecting sulfite, in particular to a method for detecting sulfite in food, and belongs to the technical field of food detection. Background technique [0002] Sulfite is a kind of food additive that has been widely used in the world for a long time: it can be used as food bleaching agent and preservative; it can inhibit non-enzymatic browning and enzymatic browning, prevent food browning, and make fruits not black It can also prevent fresh shrimp from forming black spots; it is also a very effective antibacterial agent in acidic medium. [0003] For the human body, sulfite ions are originally the products of the metabolism of sulfur-containing amino acids. Therefore, there are sulfite oxidases in the body that can oxidize them. The most abundant sulfite oxidase is in the liver. Other organs, such as It is also distributed in the heart and lungs, and also exists in the mitochondria of cells, which is an essential ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/75
Inventor 朱海军梁超雄吴婕李倩雯谭略
Owner WUZHOU INST OF PROD QUALITY INSPECTION
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