Bio-sample tomography micro-imaging system

Abstract

The invention discloses a bio-sample tomography micro-imaging system which includes a low-light imaging sub system and a sample planar processing sub system. The low-light imaging sub system is used for performing integral imaging during planar processing on the sample, and the sample planar processing sub system is used for processing the surface of the sample to form a tomography for imaging by the low-light imaging sub system. The low-light imaging sub system comprises a linear scanning module and a light spot shaping module, wherein the linear scanning module comprises a linear detector and an imaging lens. The light spot shaping module, in the direction of light path, successively comprises a beam expansion unit and a beam shrinkage unit. In the invention, by means of separation of a cutting module from the imaging system, the low-light imaging sub system is designed, so that the bio-sample tomography micro-imaging system is suitable for various resin-embedded samples. By means of separation of cutting speed from imaging speed, longitudinal low-sampling quick imaging acquisition can be carried out. In addition, the bio-sample tomography micro-imaging system can perform quick 3D information acquisition on low-light objects, such as fluorescent biological organs and the like.

Description

technical field [0001] The invention belongs to the field of biological imaging, and more specifically relates to a tomographic microscopic imaging system of biological fluorescence samples. Background technique [0002] The micro-optical tomography system is a tomographic microscopic imaging system of biological samples, which can automatically obtain relatively fine three-dimensional structure information of biological samples. The principle is as follows: the sample is mounted on a motorized platform that can move in three dimensions; strip-shaped slices can be cut out by moving the platform in the X direction, and the strip-shaped slices move along the knife face, and these slices are imaged simultaneously on the knife face. The illumination beam passes through beamsplitters, mirrors, and objectives, finally illuminating the slice. The slices are imaged on a line-array CCD through an objective lens and a tube lens. The quality of imaging, the quality of slices and the ...

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Problems solved by technology

[0004] In view of the above defects or improvement needs of the prior art, the present invention provides a tomographic microscopic imaging system for biological samples, the purpose of which is to separate the imaging module from the cutting module, process the sample first, and then process the processed sample surface At the same time, a low-light imaging system was designed to solve the limitations of the original system applicable to samples, the mutual restriction of slice speed and imaging speed, the inability to vertically low sampling, low-light imaging, especially fluorescence imaging. The imaging speed is slow question

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