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Test strip for testing mycoplasma capricolum subsp.capripneumonia and preparation method of test strip

A technology of mycoplasma and test strips, applied in the field of colloidal gold test strips for the detection of mycoplasma goat pneumonia subspecies, can solve problems such as difficulties, and achieve the effects of low cost, increased specificity, and short detection time

Inactive Publication Date: 2015-08-12
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past few years, several methods have been established for this pathogen, including the serological detection method indirect hemagglutination and the etiological detection method PCR, in which indirect hemagglutination requires professional technicians to operate and detect, and the test conditions are relatively strict; while the etiological detection method PCR needs to obtain diseased biological samples from sick sheep, which is relatively difficult and requires more stringent test conditions

Method used

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  • Test strip for testing mycoplasma capricolum subsp.capripneumonia and preparation method of test strip
  • Test strip for testing mycoplasma capricolum subsp.capripneumonia and preparation method of test strip
  • Test strip for testing mycoplasma capricolum subsp.capripneumonia and preparation method of test strip

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Embodiment Construction

[0036] The present invention is illustrated below in conjunction with examples.

[0037] The preparation method of the Mycoplasma capricum goat pneumonia subspecies antibody detection test strip of the present invention is:

[0038] one, Antigen Preparation of Whole Bacteria of Mycoplasma capricosum subsp. goat pneumoniae

[0039] The preparation method of polyclonal antibody antigen is as follows: (1) Preparation of whole bacterial antigen: Take the seeds of Mycoplasma capricum subsp. goat pneumoniae subsp. The culture was centrifuged at 12000r / min for 30min. The supernatant was discarded, the precipitate was washed twice with 0.15M PBS pH7.2, ultrasonically broken three times, centrifuged at 5000r / min for 20min, and the supernatant was taken to obtain the required whole bacterial antigen of Mycoplasma capricosum subsp. goat pneumoniae. Determination of concentration, standby;

[0040]The preparation method of the MTB medium used is as follows:

[0041] Element

[0042...

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Abstract

The invention discloses a test strip for testing mycoplasma capricolum subsp.capripneumonia and a preparation method of the test strip. The test strip is characterized in that an antigen enveloped by a colloidal gold pad is a mycoplasma capricolum subsp.capripneumonia whole cell antigen obtained after ultrasonic wave disruption, a test line envelops a capricolum subsp.capripneumonia polysaccharide antigen, and a quality control line is mycoplasma capricolum subsp.capripneumonia whole-cell poly-IgG (immunoglobulin G). The test strip can test antibodies generated by the mycoplasma capricolum subsp.capripneumonia really, has specificity of being not in cross reaction with any other similar mycoplasmas and has the advantages of low cost, simplicity in operation, long-term storage and no toxicity and harm to human bodies.

Description

technical field [0001] The invention relates to a test strip for detecting livestock diseases used in animal husbandry and a preparation method thereof, in particular to a colloidal gold test strip for detecting mycoplasma capricum subspecies goat pneumonia, and a preparation method of the test strip. Background technique [0002] Mycoplasma goat pneumoniae subspecies is the main pathogen that causes goat infectious pleuropneumonia. Goat infectious pleuropneumonia has brought great harm to the goat breeding industry, because the pathogen is very easy to be mixed with mycoplasma goat pneumoniae subspecies , and Mycoplasma goat pneumonia subspecies is difficult to separate, so early serological diagnosis has become a very important way. In the past few years, several methods have been established for this pathogen, including the serological detection method indirect hemagglutination and the etiological detection method PCR, in which indirect hemagglutination requires professio...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/543
Inventor 贺英储岳峰赵萍高鹏程逯忠新张建军刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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