vip3a-1 gene of bacillus thuringiensis expression protein with high toxicity for asparagus caterpillar

Abstract

The invention discloses a vip3A-1 gene of a bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars, and belongs to the technical field of biochemistry. The expression protein is adopted for primary screening and secondary screening on bioactivity of asparagus caterpillars, the number of dead and live asparagus caterpillars after 7 days, the death rate and LC50 are calculated and corrected, the result shows that the medial lethal concentration of the vip3A-1 gene of the bacillus thuringiensis expression protein to asparagus caterpillars is 7.755mu g / g (5.683-9.979mu g / g) in secondary screening, and when the concentration of the expression protein is up to 81mu g / g, all asparagus caterpillars can be dead, so that the vip3A-1 gene of the bacillus thuringiensis expression protein has high toxicity for asparagus caterpillars, and is characterized in that the nucleotide sequence of the vip3A-1 gene of the bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars is as shown in SEQ ID NO:1.

Description

technical field [0001] The invention relates to a vip3A-1 gene expressed by Bacillus thuringiensis which has high toxicity to beet armyworm and belongs to the technical field of biochemistry. Background technique [0002] Bacillus thuringiensis (Bt for short) belongs to the second category, Group 18 in the ninth edition of "Bergey's Bacteria Identification Manual", Gram-positive, and a species of Bacillus. During the formation of spores, one or more parasporal crystals with the same or different shapes are formed at one or both ends of the bacterium. The main component of this parasporal crystal is a protein with insecticidal activity, so it is also called insecticidal crystal protein (ICPs) or δ-endotoxin (δ-endotoxin). This insecticidal crystal protein is coded by cry gene and cyt gene. Now it has been found that most bacterial strains of Bacillus thuringiensis can produce multiple types of crystal protein, which is effective for Lepidoptera, Diptera, Coleoptera ( Coleop...

AI Technical Summary

Problems solved by technology

In recent years, beet armyworms have been rampant, causing outbreaks and disasters. Many farmers frequently use pesticides but the control effect is not good, so they have to go to the fields to catch them every morning and evening.

For example, the rate of damaged plants in general cotton fields is about 60%, and the serious plots reach 100%. Once it happens, cotton farmers are often at a loss, which directly affects the economic benefits of cotton farmers and poses a serious threat to cotton production.

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