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Nocardia braziliensis culture medium suitable for pleural effusion

A Nocardia and pleural effusion technology, applied in the field of Nocardia brasiliensis selection medium, can solve the problems of slow growth, low positive rate, and increased fatality rate, and achieve high isolation rate and reliable detection Effect

Inactive Publication Date: 2015-08-19
孙霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The primary culture of Nocardia brasiliensis grows slowly. Pleural effusion specimens are easily covered by Candida albicans, Staphylococcus aureus and other fungi and miscellaneous bacteria on blood agar medium. Nocardia brasiliensis cannot grow, and the positive rate is low. , 60% of Nocardia brasiliensis was missed by using the traditional method of laboratory culture for 48 hours, and it took about 1 week to make a more accurate identification after more than 96 hours of culture, and the biochemical identification required 4 to 6 weeks. Delayed time, causing multiple lesions to metastasize and increasing the fatality rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, the formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 2.0g; yeast extract powder 5.0g; sodium chloride 5.0g; agar 15.0g; ferrous lactate 0.01g; zinc gluconate 0.05g of calcium; 0.05g of nalidixic acid; 0.001g of chrysanthemum; 0.1g of asparagine; 100ml of sterile defibrinated sheep blood; distilled water was added to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; zinc calcium gluconate; Cool to 45°C, add sterilized nalidixic acid; mix with sterile defiberized sheep blood, dilute to 1000ml with sterilized distilled water, and dispense.

Embodiment 2

[0024] Example 2, the formula for preparing 1000ml medium contains: beef liver powder 10.0g; malt extract powder 5.0g; yeast extract powder 8.0g; sodium chloride 5.0g; agar 15.0g; ferrous lactate 0.01g; zinc gluconate 0.03g of calcium; 0.02g of nalidixic acid; 0.003g of cloverone; 0.01g of asparagine; Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; zinc calcium gluconate; , cooled to 45°C, added sterilized nalidixic acid; sterilized colulin phosphopeptide; sterilized defibrinated sheep blood, mixed evenly, sterilized distilled water to 1000ml, and then packed.

Embodiment 3

[0025] Example 3, the formula for preparing 1000ml medium contains: beef liver powder 15.0g; malt extract powder 3.0g; yeast extract powder 10.0g; sodium chloride 5.0g; agar 20.0g; ferrous lactate 0.02g; zinc gluconate Calcium 0.05g; Nalidixic acid 0.05; Trichosanone 0.005g; Asparagine 0.05g; Vitamin K 1 0.01g; sterile defibrated sheep blood 90ml; add distilled water to 1000ml. Preparation method Weigh bovine liver powder; malt extract powder; yeast extract powder; sodium chloride; agar; ferrous lactate; zinc calcium gluconate; , cooled to 45°C, added sterilized nalidixic acid; sterilized vitamin K 1 ; Mix the aseptic defibrated sheep blood evenly, dilute it with sterilized distilled water to 1000ml, and dispense it.

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PUM

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Abstract

The invention discloses a Nocardia braziliensis culture medium suitable for pleural effusion, belonging to the field of inspection microbe culture. The invention is characterized in that every 1000ml of culture medium contains ox liver powder, malt extract powder, yeast extract powder, sodium chloride, agar, ferrous lactate, zinc calcium gluconate, nalidixic acid, argutone, asparamide, sterile defibered sheep blood, casein phosphopeptide and the balance of distilled water. Compared with the prior art, the culture medium disclosed by the invention has the characteristics of high detection reliability and high separation rate of Nocardia braziliensis.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a Nocardia brasiliensis selection medium for pleural effusion specimens. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Nocardia braziliensis (N.braziliensis) is an aerobic soil saprophytic bacterium, belonging to the genus Nocardia, actinomycetes, the bacterium widely exists in air, dust, soil and livestock body surface, the toxicity of Nocardia braziliensis Strong, strong pathogenicity, can cause outbreaks, mostly through the respiratory system or primary infection, causing chronic suppurative infection of subcutaneous tissue, lung, brain and other parts. Nocardia brasil...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 孙霞
Owner 孙霞