Pig litter size-related CNV marker and use thereof

A technology of litter size and live litter size, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of unsuitable high-throughput detection, etc., so as to improve the economic benefits of pig raising, Easy to operate, accurate and reliable method

Active Publication Date: 2015-08-19
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the advantages of simple operation and low cost, and can detect the absolute copy number of the target fragment, but it is not suitable for high-throughput detection of large samples

Method used

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  • Pig litter size-related CNV marker and use thereof
  • Pig litter size-related CNV marker and use thereof
  • Pig litter size-related CNV marker and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Obtaining of CNV markers related to pig litter size

[0037] 1. Genomic DNA extraction from pig ear tissue

[0038]In this experiment, the blood and tissue DNA extraction kit purchased from QIAGEN was used to extract genomic DNA from pig ear tissue. The specific method is as follows:

[0039] 1) Take ear tissue less than 25mg and place it in a 2ml centrifuge tube, and cut it into pieces with surgical scissors;

[0040] 2) Add 180 μl buffer ATL and 20 μl proteinase K, vortex to mix, and digest at 56°C for 2-4 hours (mix once every 0.5 hours) until the tissue block is completely digested;

[0041] 3) Add 200 μl buffer AL, vortex to mix, and digest at 56°C for 10 minutes;

[0042] 4) Add 200 μl of absolute ethanol, and vortex to mix;

[0043] 5) Pour the digestion solution into the adsorption column, place in a 2ml centrifuge tube, and centrifuge at 8000rpm for 1min;

[0044] 6) Take out the adsorption column and place it in a new 2ml centrifuge tube, add 500...

Embodiment 2

[0058] Example 2 Correlation Analysis and Detection Application of Pig Different Copy Number Variations and Litter Size

[0059] According to the method of Example 1, a total of 944 sows (484 Large White, 259 Changbai, 172 Duroc and 29 Meishan) collected from Wangzu breeding pig farm in Xingtai, Hebei and Beijing Fangshan Liuma breeding pigs were used as test materials , taking the copy number variation site (chr9: 95510416..95542790) in the region of the AHR gene (such as the sequence shown in NC_010451.3) as a candidate region, and using real-time fluorescent quantitative PCR technology to detect the copy number variation of this site in the population. The results showed that there were copy number variations in the candidate region of the pig AHR gene, and the variation range was 1-2, showing normal copy number type and copy number deletion type.

[0060] Multiple comparisons of the AHR gene copy numbers among the four breeds found that there were significant differences i...

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Abstract

The invention provides a pig litter size-related CNV marker and a use thereof. The CNV marker is a copy number variant of a pig AHR gene (GenBank: NC_010451.3) candidate region chr9: 95510416..95542790, the copy number in this region is 1 or 2, correspondingly, the pig is a copy number loss type or a normal type. Association analysis of large white pig breeding properties and copy number proves that different copy numbers of the AHR gene produce substantial influence on the pig litter size, the total litter size and live litter size of the individual with the copy number of 2 are greatly higher than those of the individual with the copy number of 1 (P is less than 0.01). The invention also provides primers for detecting the CNV marker and a kit containing the primers. The invention also provides a use of the CNV marker in identification of the types of pigs with high litter size. The CNV can be used as a very important candidate molecular marker for marker-assistant selection of large white pig litter size properties.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a CNV marker related to pig litter size and application thereof. Background technique [0002] Pig reproductive traits are important economic traits in modern pig production, and the level of reproductive performance directly affects the production efficiency and economic benefits of pig farms. At the same time, reproductive traits are quantitative traits with low heritability and are easily affected by environmental conditions. They mainly include litter size, live litter size, birth weight, litter weight at birth, litter weight at 20 days, number of weaned cows, and weaned weight How to improve the reproductive performance of sows is the key to the healthy, efficient and sustainable development of the pig industry, and it is also the focus and hot spot of current genetic breeding research. Studying the copy number variation of genes related to pig reproduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 刘剑锋杨凯捷郑先瑞赵鹏举王海飞
Owner CHINA AGRI UNIV
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