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Detecting method for content of linderane in Lindere.aggregata(Sims)Kosterm

An agaric ether lactone and determination method technology, applied in the field of chemical detection, can solve the problems of long test time, large solvent consumption, and high skill requirements of testers, so as to reduce the extraction time, reduce the solvent consumption, and solve the problem of low extraction efficiency. Effect

Active Publication Date: 2015-08-19
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The determination method of the content of araegina lactone in the above-mentioned black medicine uses a large amount of solvent, takes a long time to test, and requires high skills of testers

Method used

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  • Detecting method for content of linderane in Lindere.aggregata(Sims)Kosterm
  • Detecting method for content of linderane in Lindere.aggregata(Sims)Kosterm
  • Detecting method for content of linderane in Lindere.aggregata(Sims)Kosterm

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Embodiment 1

[0026] extraction

[0027] The instruments and consumables used in this embodiment are: Pulverizer, Electronic Analytical Balance (XA205DU): Sensitivity 0.001g, Accelerated Solvent Extractor Model: ASE-350 (10ml extraction pool, 50ml collection bottle), volumetric bottle: 25ml, Centrifuge tube: 2ml.

[0028] Step 1: Take about 1g of black herb powder (passed through a No. 3 sieve), weigh it accurately, mix it with 1g of diatomaceous earth, and set aside;

[0029] Step 2: Pad 3 to 6 pieces of homemade round filter paper (diameter 27mm) at the bottom of the extraction pool (1 piece of commercial filter paper), add about 1g of diatomaceous earth, then add the homogeneously mixed sample obtained in step 1, and then add an appropriate amount of diatomaceous Shake it gently to make it on the same level as the mouth of the pool, and tighten the upper cover of the extraction pool.

[0030] Step 3: extract and extract with ASE to obtain the extract, the specific parameters are shown ...

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Abstract

The invention discloses a detecting method for the content of linderane in Lindere.aggregata(Sims)Kosterm, which comprises the following steps: step 1) pulverizing the Lindere aggregata and mixing the pulverized Lindere aggregate with diatomite; step 2) extracting linderane by adopting an ASE extraction method, collecting the extract, wherein extracting solvent adopted according the ASE extraction method is mixed solution of ethyl acetate and n-hexane, and the volume ratio of the ethyl acetate to the n-hexane is 1:1; step 3) after drying the extract, dissolving the extract by using methanol, diluting, determining the volume, centrifuging and taking supernatant; step 4) detecting the supernatant by using an HPLC (High Performance Liquid Chromatography). The invention aims to provide the detecting method for the content of linderane in Lindere.aggregata(Sims)Kosterm, which is high in extraction efficiency and accurate in detection.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a method for determining the content of agragenolactone in black medicine. Background technique [0002] Aegregia is the dry tuber root of Lauraceae plant Aegregata Lindere. aggregata (Sims) Kosterm_. Can be excavated throughout the year, remove fine roots, wash, slice while fresh, and dry in the sun, or directly in the sun. Most of the black herbs are spindle-shaped, slightly curved, and the middle part of some shrinks into a bead shape, with a length of 6-15 cm and a diameter of 1-3 cm. The surface is yellowish brown or yellowish brown, with longitudinal wrinkles and sparse fine root marks. Quality is hard. The slices are 0.2-2mm thick, the cut surface is yellowish-white or light yellowish-brown, the rays are radial, annual rings can be seen, and the center is darker. The gas is fragrant, the taste is slightly bitter, pungent, and has a refreshing feeling. Tapro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 陈学松欧妮
Owner WUZHOU INST FOR FOOD & DRUG CONTROL