Building method of mouse model for expressing human NTCP (Na+/taurocholate Cotransporting Polypeptide) as hepatitis b virus receptor

A construction method and mouse model technology, which can be applied in other methods of inserting foreign genetic materials, recombinant DNA technology, animal husbandry, etc., can solve the problem of lack of continuous and stable expression of human NTCP mouse model, lack of expression of human NTCP mouse model and other issues, to achieve the effect of advanced evaluation system, technology platform, and rich research methods
CN104862336AInactive Publication Date: 2015-08-26中国人民解放军第四五八医院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中国人民解放军第四五八医院
Publication Date
2015-08-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a building method of a mouse model for expressing human NTCP (Na+ / taurocholate Cotransporting Polypeptide) as a hepatitis b virus receptor. A human NTCP gene carried micro ring carrier is built and introduced into a mouse body in a hydrodynamic transfection type transgenic mode so as to enable human NTCP genes to be expressed in a mouse liver. According to the building method of the mouse model for expressing the human NTCP as the hepatitis b virus receptor, the defect that the existing experimental mouse cannot be naturally infected with an HBV (Hepatitis B Virus) is overcome due to the mouse model, the mouse model comprising the HNV functional receptor is built, the HBV in-vivo infection can be supported, the normal immune function is achieved, and accordingly the clinical HBV occurrence, development and chronic process can be really and fully reflected and the ideal animal model is provided for the further research on a pathogenic mechanism of the HBV, the pathological changes of the engine body after the HBV infection and an action mechanism of an engine body immune system in the process of antiviral infection and immune pathogenesis.
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Description

Technical field

[0001] The present invention is a technical field of animal model preparation, which involves a method of building a mouse model that expresses hepatitis B virus receptor -human NTCP. Background technique

[0002] Hepatitis B Virus (HBV) infection is a major issue that affects human health globally.Chronic hypomential inflammatory lesions caused by HBV infection often develop to liver cirrhosis, liver cancer and eventually lead to death.According to the World Health Organization, about 2 billion people around the world have infected HBV and currently about 350 million people are chronic infected by HBV. These people have progressing the risk of cirrhosis, liver failure or liver cancer.30%of the global cirrhosis and more than 50%of liver cancer are caused by HBV infections. The number of deaths caused by HBV infections is about 1 million to 2 million each year.Liver disease caused by HBV infection is still a major public health problem that needs to be solved.For a...

Claims

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