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Extraction and purification method of total flavonoids and craneol in Aralia japonica

A kind of purification method, the technology of geniol, which is applied in the field of extraction and purification of total flavonoids and geniol in A.

Inactive Publication Date: 2016-06-15
HUNAN CITY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] One of the purposes of the present invention is to solve the problem that only one substance can be extracted and purified at a time in the above-mentioned prior art, and to provide a method for extracting and purifying total flavonoids and cretinol respectively through two Soxhlet extraction methods, Saving the utilization rate of two kinds of active active ingredients in the unit mass of Aralia japonicus

Method used

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  • Extraction and purification method of total flavonoids and craneol in Aralia japonica
  • Extraction and purification method of total flavonoids and craneol in Aralia japonica
  • Extraction and purification method of total flavonoids and craneol in Aralia japonica

Examples

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Embodiment 1

[0037] Soxhlet Extraction of Total Flavonoids in Agrimony

[0038] Put the agrimony in an oven, treat it in the oven at 100°C for 10 hours to a constant weight, pulverize it and pass through a 100-mesh sieve to obtain agrimony powder. Take 50g of Agrimonia vulgaris powder with pure ethanol as the reflux liquid, reflux in a 500mL Soxhlet extractor for 4 hours, and concentrate the reflux solution to obtain 7.26g Agrimonia vulgaris flavonoid extract. figure 1 The flow chart of the extraction and purification of total flavonoids and hortifenol in Agrimonia vulgaris in this example is shown.

Embodiment 2

[0040] Soxhlet Extraction of Agrimonia vulgaris

[0041] Keep the agrimony powder in the Soxhlet extractor from the previous step unchanged, replace the Soxhlet extractor flask (500mL) with benzene, perform a second Soxhlet extraction, the extraction time is 5h, and reflux The liquid was concentrated to obtain 4.22 g of horchonol extract. figure 2 Shown in this example is the molecular structure diagram of griffinol.

Embodiment 3

[0043] Purification of Total Flavonoids in Agrimony

[0044] 4.00g of Agrimonia vulgaris flavonoid extract was mixed with 4mL85% ethanol to form a concentrated solution, passed through a model S-8 macroporous adsorption resin column, with a flow rate of 2.5BV / h with 45% ethanol solution and 70% acetone solution The mixed solution is used as the eluent for elution until there is no flavonoids in the eluent, the eluent is collected, concentrated, and dried to obtain 2.934 g of yellow agrimony flavonoids (I).

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Abstract

The invention belongs to the field of purification of active components of Chinese traditional herbs, and discloses a method for extracting and purifying general flavones and agrimophol in hairyvein agrimony. The method comprises the following steps: after hairyvein agrimony is taken and dried, sieving the hairyvein agrimony, and carrying out Soxhlet extraction on powder so as to obtain a hairyvein agrimony flavonoid extract; keeping the powder unchanged and replacing a return fluid, so that a hairyvein agrimony agrimophol extract can be extracted; enabling the obtained hairyvein agrimony flavonoid extract to pass through a macroporous adsorption resin column to elute, condensing eluent, and drying the eluent, so that a hairyvein agrimony flavonoid is obtained; preparing the obtained hairyvein agrimony agrimophol extract into a concentrated solution, enabling the concentrated solution to pass through a macroporous adsorption resin column, after the concentrated solution is eluted until the solution is colourless, recrystallizing eluant, and drying the eluant, so that agrimophol crystals are obtained. According to the invention, general flavones and agrimophol in hairyvein agrimony are separated by using a two-step Soxhlet extraction method, so that extracts of general flavones and agrimophol are respectively obtained, and in combination with a macroporous adsorption resin method, the extracts of general flavones and agrimophol are purified, so that two products, i.e. high-purity hairyvein agrimony flavonoid and agrimophol are obtained. The method effectively improves the utilization rate of active ingredients in hairyvein agrimony.

Description

Technical field [0001] The invention belongs to the field of purification of active ingredients of traditional Chinese medicine, and specifically relates to a method for extracting and purifying total flavonoids and gleninol in Agrimony. Background technique [0002] Agrimonia (Agrimonia.Pilosaledeb) is the whole plant of Agrimonia.Pilosaledeb Varjaponica (Miq) Nakai (Miq) Nakai, also known as Tuoli grass, Agrimonia. Mainly distributed in China, North Korea, Mongolia, Japan, Russia, it is a commonly used Chinese medicine. Agrimony is rich in nutrients and physiologically active ingredients. The whole herb contains Agrimvnias (Agrimvnias). It is known to have Agrimvnia A, Agrimvnia B, Agrimvnia C, and Agrimvnias. There are six kinds of glutinin, agrimony and agrimony, and there are still tannins, sterols, saponins and volatile oils. According to the structure classification, it can be divided into: pyrogallol condensate, flavonoids, organic acids, lactones and coumarins, as well...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C45/78C07C45/79C07C45/80C07C49/84A61K36/73A61P35/00
CPCA61K36/73C07C45/79C07C45/80C07C49/84
Inventor 贺国文龙立平肖谷清吴丹张建国
Owner HUNAN CITY UNIV