A liquid preparation produced from livestock and poultry breeding wastewater and its application in the prevention and removal of continuous cropping obstacles

A technology of livestock and poultry breeding wastewater and liquid preparations, which is applied in the field of microorganisms, can solve problems such as no discovery, and achieve the effect of reducing costs and reducing environmental pollution

Active Publication Date: 2017-11-03
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no literature reports related to the subject of the present invention have been found

Method used

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  • A liquid preparation produced from livestock and poultry breeding wastewater and its application in the prevention and removal of continuous cropping obstacles
  • A liquid preparation produced from livestock and poultry breeding wastewater and its application in the prevention and removal of continuous cropping obstacles
  • A liquid preparation produced from livestock and poultry breeding wastewater and its application in the prevention and removal of continuous cropping obstacles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of a liquid formulation for controlling crop continuous cropping obstacles.

[0034] (1) Bacteria production

[0035] (1) Inoculate Bacillus subtilis D9 into a Bacillus subtilis liquid fermentation medium to prepare a Bacillus subtilis fermentation broth. The conditions for its fermentation production are as follows: the initial pH range is 6.5-7.2, the culture temperature is 35-37°C, the dissolved oxygen ventilation range is 30-100%, 120-180rpm, and ferment for 18 hours to obtain the Bacillus subtilis D9 fermentation liquid; wherein, The liquid fermentation medium used is measured in a volume of 1L, including 3g of beef extract, 10g of peptone, 3g of NaCl, and 1000ml of tap water, with a pH range of 7.0 to 7.5, and is used after being sterilized at 121°C for 20 minutes.

[0036] (2) Inoculate Trichoderma aculeatus 12 into PDA culture fluid, carry out liquid fermentation production, obtain Trichoderma aculeatus 12 fermentation broth, the condition...

Embodiment 2

[0041] Example 2 Preparation of a liquid formulation for controlling crop continuous cropping obstacles.

[0042] (1) Bacteria production

[0043] (1) Inoculate Bacillus subtilis D9 into a Bacillus subtilis liquid fermentation medium to prepare a Bacillus subtilis fermentation broth. The conditions for its fermentation production are as follows: the initial pH range is 6.5 to 7.2, the culture temperature is 35 to 37°C, the dissolved oxygen ventilation range is 30 to 100%, 120 to 180 rpm, and 16 hours of fermentation to obtain the Bacillus subtilis D9 fermentation liquid; wherein, The liquid fermentation medium used is measured in a volume of 1L, including 3g of beef extract, 10g of peptone, 3g of NaCl, and 1000ml of tap water, with a pH range of 7.0 to 7.5, and is used after being sterilized at 121°C for 20 minutes.

[0044] (2) Inoculate Trichoderma aculeatus 12 into PDA culture fluid, carry out liquid fermentation production, obtain Trichoderma aculeatus 12 fermentation bro...

Embodiment 3

[0049] Example 3 The application of the liquid preparation for preventing continuous cropping obstacles of the present invention on the land with continuous cropping obstacles of Artemisia annua.

[0050] The experimental site is located in Qixia District, Nanjing, Jiangsu Province. Artemisia annua has been planted in the soil continuously for 5 years. In the fourth year, the incidence of Artemisia annua in winter and spring is 9%, and in the fifth year, the incidence of Artemisia annua in winter and spring is 100%.

[0051] Three treatments were set up in the experiment, namely:

[0052] (1) Control treatment: conventional planting;

[0053] (2) Treatment of aquaculture wastewater: Continuous cropping soil application of aquaculture wastewater 5 tons / mu;

[0054] (3) Product treatment of the present invention: 5 tons / mu of the product of the present invention (the liquid preparation for preventing and eliminating continuous cropping obstacles prepared by embodiment 1) in con...

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PUM

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Abstract

The invention discloses a liquid preparation produced by the utilization of livestock and poultry breeding wastewater and an application of the liquid preparation in controlling and eliminating continuous cropping obstacles. The liquid preparation is prepared from bacillus subtilis D9 and Trichoderma asperellum 12 fermentation livestock and poultry breeding wastewater. Total microorganism amount in the liquid preparation is greater than 10*10<8>cfu ml<-1>. Effective functional microorganism colony-forming unit is greater than or equal to 0.5*10<7>cfu ml<-1>. Content of bacillus subtilis D9 is greater than 0.5*10<7> cfu ml<-1>. Content of Trichoderma asperellum 12 is greater than 0.1*10<6> cfu ml<-1>. Amount of biocontrol microorganisms around rhizosphere of seedlings of cutting tissue or transplanted crops, pathogen-infected cutting tissue or transplant seedlings in continuous cropping soil are minimized, continuous cropping obstacles are effectively controlled and eliminated, and a lot of breeding wastewater can be consumed. It shows through tests that incidence of disease is reduced by 93.8% after the production is applied on artemisia seleirgensis continuous cropping obstacle soil; 5-8 tons of breeding wastewater can be consumed per mu of artemisia seleirgensis continuous cropping land; and incidence of disease is reduced by 87.3% after the production is applied on tomato continuous cropping soil, and 10-20 tons of breeding wastewater can be consumed per mu of tomato planting land.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a liquid preparation produced by utilizing livestock and poultry breeding wastewater and its application in preventing and eliminating continuous cropping obstacles. Background technique [0002] With the development of society, livestock and poultry breeding has become multi-enterprise operation and intensive breeding, with high breeding density and reduced unit breeding cost. However, intensive farming has resulted in a large amount of waste from livestock and poultry farming. The solid waste can be composted to form a solid product that can be transported to other places for long-distance use. However, the large amount of waste water produced cannot be absorbed by the surrounding farmland in time, and it is transported to long-distance farmland in the form of fertilizer. The transportation cost is too high, and the enthusiasm of breeding enterprises and grow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/04A01P21/00A01G1/00A01B79/02C12R1/125C12R1/885
Inventor 陈立华常义军王长春邵孝候常婷婷黄忠阳
Owner HOHAI UNIV
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