Citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit

Abstract

The invention discloses a citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit. By means of the citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit, a pair of primers is designed according to the citrobacter freundii 16S rRNA gene sequence in a GenBank; a fluorescent quantitative PCR reaction condition is optimized, and the citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit is developed. The citrobacter freundii fluorescent quantitative PCR diagnostic reagent kit is used for testing clinical samples simply, conveniently, quickly, sensitively and accurately, and a scientific basis is provided for prevention and treatment of the disease. The studied and designed primers are applied to a fluorescent quantitative PCR rapid test kit, so that citrobacter freundii is quickly tested, and good specificity and sensitivity are achieved.

Description

technical field [0001] The invention relates to a kit, in particular to a fluorescent quantitative PCR diagnostic kit for Citrobacter freundii. Background technique [0002] Citrobacter freundii ( Citrobacter freundii ) is a Gram-negative short bacillus that widely exists in soil, water, sewage, and food. It is a normal flora in the intestinal tract of humans and animals. septicemia [1-3] . In recent years, the cultivation of cold-water fish in Guizhou Province has developed rapidly. The main species are rainbow trout, golden trout, spotted trout, sturgeon, and giant salamander. As the intensification of cold-water fish farms (households) continues to increase, the amount of seed circulation and exchange continues to increase, and the number of diseases is gradually increasing, and the disease is becoming more and more complicated. The disease reports currently reported are mainly concentrated on sturgeon, shrimp, Chinese soft-shelled turtle, trout, salmon, and giant sal...

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