Discrimination method for cashmere and wool, and marker peptide segment used for method

A technology of markers and wool, which is applied in the field of identification of marker peptides, cashmere and wool, can solve the problems of inability to distinguish other types of fibers, high experimental costs, wool confusion, etc., and achieve strong detection effectiveness, high sensitivity, and stability Good results

Inactive Publication Date: 2015-09-09
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of detection method requires inspectors to have contact with a large number of cashmere samples and accumulate decades of practical experience, which is highly subjective.
In addition to the most commonly used optical microscope detection method, foreign researchers have also tried to use scanning electron microscopy to observe the surface morphology and fine structure of textile fibers, but this method is expensive and cumbersome to operate, and it cannot be distinguished from cashmere fibers that are very similar in shape and structure. other types of fibers, among which the confusion of wool is the most prominent
In addition, researchers also identify cashmere components by analyzing lipid molecules and DNA sequence differences in fiber materials or preparing antibodies that specifically recognize cashmere. Such exploratory methods are often subject to various chemical treatments in the production process (such as bleaching, Staining) interference, making the results biased

Method used

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  • Discrimination method for cashmere and wool, and marker peptide segment used for method
  • Discrimination method for cashmere and wool, and marker peptide segment used for method
  • Discrimination method for cashmere and wool, and marker peptide segment used for method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 (the specificity experiment of cashmere, wool composition identification method)

[0034] 1. Materials

[0035] Standard cashmere fiber samples, standard wool fiber samples and standard yak wool fibers are provided by Tianjin Textile Engineering Research Institute.

[0036] 2. Method

[0037] Take the cashmere fiber standard product, the wool fiber standard product and the yak wool fiber and mix them in pairs. The above-mentioned mixed samples, standard cashmere fiber samples, standard wool fiber samples, and standard yak wool fiber samples were used as test substances, the total protein was extracted, and the signature peptide QCCESNLEPLFSGYIETLR and the signature peptide YEEEVALR were detected by biological mass spectrometry analysis technology.

[0038] (1) Protein extraction from textile fiber samples:

[0039] The textile fiber samples were shredded, soaked in ethanol, and soaked in chloroform-methanol solution. After the soaking solution was filte...

Embodiment 2

[0046] Embodiment 2 (sensitivity experiment of cashmere, wool component marker protein identification)

[0047] 1. Materials

[0048] Cashmere fiber standard products, wool fiber standard products, yak wool fiber standard samples.

[0049] 2. Method

[0050] Take standard cashmere and standard wool fiber samples and mix them with yak wool fiber standard samples in different proportions to obtain 6 samples with cashmere content of 5%-50% and wool content of 5%-50% respectively, and extract the total protein. Detect cashmere signature peptide QCCESNLEPLFSGYIETLR and wool signature peptide YEEEVALR.

[0051] 3. Results

[0052] Such as Image 6 , 7 As shown in Table 1, based on the analysis of the cashmere signature peptide QCCESNLEPLFSGYIETLR, 5% cashmere components ( Image 6 ). Similarly, based on the analysis of the wool signature peptide YEEEVALR, the wool component with a content of 5% ( Figure 7 ). The sensitivity test results show that the sensitivity of the est...

Embodiment 3

[0055] Embodiment 3 (identification analysis of cashmere, wool in test material)

[0056] 1. Materials

[0057] Commercially available cashmere material S1, S2, S3, commercially available wool material S4, identified by the fiber fineness meter, S1, S2, are all cashmere and wool blended clothing materials, S3 is cashmere, yak wool blended clothing material, S4 is cashmere, camel hair blended clothing material Clothing.

[0058] 2. Method

[0059] Each test product was processed separately, the total protein was extracted, and the cashmere signature peptide QCCESNLEPLFSGYIETLR and the wool signature peptide YEEEVALR were detected.

[0060] 3. Results

[0061] Such as Figure 8 , 9 As shown in Table 2, the cashmere signature peptide QCCESNLEPLFSGYIETLR and the wool signature peptide YEEEVALR in the test material S1 and the test material S2 are all positive, indicating that the two test materials contain cashmere and wool ingredients. Only the cashmere signature peptide QCC...

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PUM

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Abstract

The invention provides a discrimination method for cashmere and wool and a marker peptide segment used for the method. The discrimination method is a detection technology directed at a specific protein molecular marker; the marker has strong specificity, good stability and high sensitivity and is not interfered by physical and chemical treatment like bleaching and dyeing in production process, so directivity of the marker to cashmere and wool is stable and reliable; and with the method, validity of discrimination and detection of cashmere and wool is high. With the discrimination method and the marker peptide segment, one can rapidly, accurately and objectively determine whether a plush textile contains cashmere and wool or not, and scientific and reliable detection means is provided for quality inspection of the plush textile.

Description

technical field [0001] The invention relates to the technical field of textile detection, in particular to a method for identifying cashmere and wool and a marker peptide used in the method. Background technique [0002] Cashmere is a rare special animal fiber, known as "fiber diamond" and "soft gold". China is the world's largest cashmere producer, and cashmere production accounts for about 75% of the world's total. China is also the world's largest exporter of cashmere, with an annual export volume of cashmere and its products exceeding US$1.5 billion. At the same time, the domestic market for cashmere products is also very broad. [0003] Driven by huge economic interests, the phenomenon of adulteration and counterfeiting in the industry has repeatedly appeared, which seriously hinders the healthy, benign and sustainable development of the cashmere industry. Most of the mixed adulteration of cashmere textile fiber products in the market today is to mix low-value raw mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02C07K7/06C07K7/08
Inventor 水雯箐曹琦琛王霁华张志丹李珊珊
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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