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Plant self nitrogen fixation by mimicking prokaryotic pathways

A nitrogen fixation and plant technology, applied in plant products, botany equipment and methods, angiosperms/flowering plants, etc., can solve problems such as failure

Inactive Publication Date: 2015-09-09
阿迪·查尔兹曼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Several unpublished attempts were made to extend the 'host' range of rhizobia, all of which failed

Method used

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  • Plant self nitrogen fixation by mimicking prokaryotic pathways
  • Plant self nitrogen fixation by mimicking prokaryotic pathways
  • Plant self nitrogen fixation by mimicking prokaryotic pathways

Examples

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Embodiment

[0060] Example: Generation of plasmids for tomato plastid transformation

[0061] In the present disclosure, ammonia and / or ammonium are produced in plants using prokaryotic or bacterial nitrogenase and BNF. These transgenic plants have the ability to meet all or part of their nitrogen requirements after transformation with a plasmid encoding BNF. Crops suitable for human consumption such as tomato, rice or wheat, as well as suitable horticultural plants such as flowers, grasses and trees, including other plants and algae, can also be transformed for auto / self nitrogen fixation. Since these plasmids do not exist in nature, synthetic biology was used to generate artificial plasmids capable of self / self nitrogen fixation by cells / plants. Said artificial plasmids contain modifications of the CDS and control sequences to perform new tasks such as expression and accumulation of nitrogenase in plants.

[0062] Example: Isolation of Genetic Material

[0063] Plant DNA Isolation:...

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Abstract

A method for engineering a transgenic plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria, such as cyanobacteria, or that of other bacteria, by targeting to or expressing in plant plastids bacterial nif (nitrogen fixation) genes is provided. A method for reducing the overall concentration of nitrogen in soil is provided using a plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria. Progeny of a plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria is also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 61 / 858,218, filed July 25, 2013, and U.S. Provisional Application No. 61 / 732,490, filed December 3, 2012, each of which is incorporated by reference in its entirety Incorporated into this article. technical field [0003] The present disclosure relates to a novel transgenic plant by targeting or expressing the bacterial nif (nitrogen fixation) gene to produce a structural protein of nitrogenase, and by introducing the synthesis of a protein homologous to the structural protein of nitrogenase DNA sequences to replicate the nitrogen fixation machinery of photosynthetic bacteria such as cyanobacteria or other bacteria, and methods of producing such plants. Background technique [0004] Plants are living organisms that belong to the kingdom Plantae. All higher plants have the vascular tissues xylem (mainly transports water and minerals) and phloem (main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
CPCC12N15/8261C12N15/8243Y02A40/146
Inventor 阿迪·查尔兹曼
Owner 阿迪·查尔兹曼
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