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Technology for extracting pyracantha fortuneana fruit total flavonoids

An extraction process, the technology of Pyracantha, which is applied in the field of natural product extraction and separation, can solve the problems of destroying the structure of total flavonoids, reducing it, and not being suitable for industrial production, and achieving the effects of low production cost, high yield, and avoiding damage

Inactive Publication Date: 2015-09-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional reflux extraction method is mostly used for the extraction of total flavonoids from Pyracantha fruit, but the traditional water extraction or solvent reflux extraction methods have problems such as excessive temperature will destroy the structure of total flavonoids, affect its biological activity and reduce yield. , not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Put Pyracantha fruit in a homogenizer, add 50% ethanol solution 1 times the weight of the raw material, and extract the homogenate for 10 minutes; the homogenized material is placed in an ultrasonic device with a frequency of 40kHz, and then add 3 times the weight of the raw material 50% ethanol solution, extract at 70°C for 1 hour, filter, and collect the filtrate; add activated carbon with a mass volume ratio of 1:10 to the filtrate, and keep stirring, let stand and filter, collect the filtrate; concentrate the filtrate until it has no alcohol smell , transferred to the XDA-1 macroporous adsorption resin column with a diameter-to-height ratio of 1:6, first eluted with 2BV of distilled water, and then eluted with 4BV of 70% ethanol, collected the eluate, concentrated under reduced pressure, and dried to Constant weight, namely the total flavonoids of Pyracantha, the yield is 1.57%, and the total flavonoids content is 77.1%.

Embodiment 2

[0021] Put Pyracantha fruit in a homogenizer, add 60% ethanol solution of 1 times the weight of the raw material, homogenize and extract for 15 minutes; put the homogenized material in an ultrasonic device with a frequency of 40kHz, and then add 5 times the weight of the raw material 60% ethanol solution, extract at 75°C for 3 hours, filter, and collect the filtrate; add activated carbon with a mass volume ratio of 1:15 to the filtrate, keep stirring, let stand and filter, and collect the filtrate; concentrate the filtrate until it has no alcohol smell , transferred to the AB-8 macroporous adsorption resin column with a diameter-to-height ratio of 1:8, first eluted with 3BV of distilled water, and then eluted with 6BV of 75% ethanol, collected the eluate, concentrated under reduced pressure, and dried to Constant weight, namely the total flavonoids of Pyracantha, the yield is 2.01%, and the total flavonoids content is 82.5%.

Embodiment 3

[0023] Put Pyracantha fruit in a homogenizer, add 55% ethanol solution twice the weight of the raw material, homogenate and extract for 15 minutes; put the homogenized material in an ultrasonic device with a frequency of 40kHz, and then add 4 times the weight of the raw material 55% ethanol solution, extract at 80°C for 2 hours, filter, and collect the filtrate; add activated carbon with a mass volume ratio of 1:12 to the filtrate, and keep stirring, let stand and filter, collect the filtrate; concentrate the filtrate until it has no alcohol smell , transferred to a D-101 macroporous adsorption resin column with a diameter-to-height ratio of 1:7, first eluted with 3BV of distilled water, and then eluted with 5BV of 80% ethanol, collected the eluate, concentrated under reduced pressure, and dried to Constant weight, namely the total flavonoids of Pyracantha, the yield is 2.18%, and the total flavonoids content is 80.3%.

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Abstract

The invention relates to a technology for extracting pyracantha fortuneana fruit total flavonoids. The technology is characterized by comprising the following steps that firstly, a pyracantha fortuneana fruit is taken out and put into a homogenizer to be mixed and homogenized with an extraction solvent in the homogenizer; secondly, the homogenized materials are put into an ultrasonic device with the frequency of 40 kHz to be extracted for 1-3 hours and filtered, and filtrate is collected; thirdly, the filtrate is decolored through activated carbon, stirred and filtered in a standing mode, and the filtrate is collected; fourthly, the filtrate is concentrated until the alcohol smell does not exist and is purified through a macroreticular resin column, ethanol elution is conducted, eluent is collected, concentrated and dried, and the pyracantha fortuneana fruit total flavonoids is obtained. The technology has the advantages of being free of dust, simple in process, fast and high in efficiency, meanwhile, the organic solvent use amount of the whole technology is small, environmental friendliness is achieved, and the good application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of natural products, and in particular relates to an extraction process of total flavonoids of Acanthopanax fruit. Background technique [0002] Pyracantha is an evergreen shrub of the genus Pyracantha in the family Rosaceae, also known as life-saving food, rescue food, torch fruit, red sun seed, etc. It has high edible value and medicinal value. Its extract has the functions of anti-oxidation, enhancing immunity, strengthening physical strength, and lowering blood fat. , rutin and other flavonoids. Flavonoids are compounds with various biological activities such as anti-oxidation, anti-virus, anti-tumor, anti-aging and protecting liver and stomach, so they are widely used in chemical fields such as medicine and food. [0003] At present, the traditional reflux extraction method is mostly used for the extraction of total flavonoids from Pyracantha fruit, but the traditional wa...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K131/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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