The medical application of Antrodia cinnamomea ac09 in the protection of chemical liver injury
A technology of chemical liver injury and Antrodia bacterium, which is applied in the field of freeze-dried powder of fermented liquid of high-yielding strains of Antrodia cinnamomea, can solve problems such as not being able to meet the demand, achieve significant health effects, reduce industrialization costs, and have good market development prospects
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Embodiment 1
[0022] Ferment Antrodia camphorata strain AC09 in a 100-liter fermenter (medium components: peptone 10g / L, glucose 20g / L, yeast extract powder 10g / L, anhydrous magnesium sulfate 2g / L, potassium dihydrogen phosphate 2g / L L, VB 1 0.1g / L, the fermentation conditions are: the liquid volume is 80 liters, the inoculum volume (v / v) is 10%, the fermentation temperature is 26 degrees Celsius, the rotation speed of the fermentation tank is 150 rpm, and the fermentation time is 4 days). 73 liters of liquid was centrifuged at 4000 rpm for 10 minutes, the supernatant was discarded, and the precipitate was collected and freeze-dried at -40°C for 60 hours to obtain 931 grams of lyophilized powder of Antrodia camphorata fermentation broth.
Embodiment 2
[0024] Antrodia high-yield strain AC09 was fermented in a 100-liter fermenter (medium components: peptone 12g / L, glucose 18g / L, anhydrous magnesium sulfate 4.0g / L, potassium dihydrogen phosphate 4.0g / L, VB 1 0.15g / L, the fermentation conditions are: the liquid volume is 75 liters, the inoculum volume (v / v) is 5%, the fermentation temperature is 25 degrees Celsius, the rotation speed of the fermentation tank is 175 rpm, and the fermentation time is 3 days). 68 liters of liquid were centrifuged at 4000 rpm for 10 minutes, the supernatant was discarded, and the precipitate was collected and freeze-dried at -40°C for 65 hours to obtain 739 grams of lyophilized powder of Antrodia camphorata fermentation broth.
Embodiment 3
[0026] The high-yield strain AC09 of Antrodia camphorata selected by mutagenesis was fermented in a 100-liter fermenter (medium components: peptone 15g / L, glucose 25g / L, anhydrous magnesium sulfate 3g / L, potassium dihydrogen phosphate 3.0g / L , VB 1 0.15g / L fermentation conditions are: liquid volume is 70 liters, inoculum volume (v / v) 10%, fermentation temperature is 26 degrees Celsius, fermentation tank speed is 125 rpm, fermentation time is 5 days), and the fermentation liquid is obtained after the fermentation is terminated 63 liters were centrifuged at 4000 rpm for 10 minutes, the supernatant was discarded, the precipitate was collected and freeze-dried at -40°C for 65 hours to obtain 801 grams of lyophilized powder of Antrodia camphorata fermentation broth.
[0027] Understand the medical application of Antrodia camphorata strain AC09 of the present invention in the protection of chemical liver damage by the following experiments
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