Supercharge Your Innovation With Domain-Expert AI Agents!

Method for extracting endocrine disrupter

A technology of endocrine disruptors and extraction methods, applied in the preparation of test samples, etc., can solve the problems of low extraction efficiency, poor impurity removal function, poor extraction effect, etc., and achieve high extraction efficiency, removal of impurities, accurate qualitative and quantitative The effect of the analysis

Active Publication Date: 2015-09-23
FOOD INSPECTION CENT OF CIQ SHENZHEN +2
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, and provide a method for extracting endocrine disruptors, aiming to solve the problems of low extraction efficiency, poor impurity removal function and poor extraction effect in the existing methods for extracting endocrine disruptors. technical problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting endocrine disrupter

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0033] The subcritical water extraction treatment process is: take 5g solid sample and 2g diatomaceous earth, mix them uniformly and add them into a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is water with 20% acetone as a modifier, and the pH of the extraction solvent is 12. The extraction temperature was 100° C., and the extraction time was 10 min.

[0034] The DLLME treatment process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of chlorobenzene as an extraction solvent. The centrifuge tube was vortexed at 2500 rpm for 30 seconds, then centrifuged at 7000 rpm for 5 min, and the chlorobenzene at the tip of the centrifuge tube was recovered with a micro-sampling needle.

Embodiment 2

[0036] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0037] The subcritical water extraction treatment process is: take 5g solid sample and 3g diatomite, mix them uniformly and add them into a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is water with 15% ethanol as a modifier, and the pH of the extraction solvent is 11. The extraction temperature is 110° C., and the extraction time is 10 minutes.

[0038] The DLLME process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of chloroform as an extraction solvent. The centrifuge tube was vortexed at 1500 rpm for 10 seconds, then centrifuged at 6500 rpm for 8 min, and the chloroform at the tip of the centrifuge tube was recovered with a microsampling needle.

Embodiment 3

[0040] A method for extracting endocrine disruptors, wherein the sample to be tested is subjected to subcritical water extraction and then DLLME processing; details are as follows:

[0041] The subcritical water extraction treatment process is as follows: Weigh 5g solid sample, 2g diatomaceous earth and 2g neutral alumina, mix them uniformly and add them to a 10ml extraction pool for extraction treatment; wherein, the extraction solvent is 10% acetonitrile as a modifier water, the pH of the extraction solvent is 10. The extraction temperature is 120° C., and the extraction time is 10 min.

[0042] The DLLME treatment process is as follows: add the extract obtained by subcritical water extraction into a conical centrifuge tube, and then add 150 microliters of carbon tetrachloride as an extraction solvent. The centrifuge tube was vortexed at 1000 rpm for 30 seconds and then centrifuged at 6000 rpm for 10 min, and the chlorobenzene at the tip of the centrifuge tube was recovered...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for extracting an endocrine disrupter. The method for extracting the endocrine disrupter comprises the following steps of performing subcritical water extraction on a to-be-detected sample, and performing dispersive liquid-liquid micro-extraction on the sample. According to the method, the subcritical water extraction technology and the dispersive liquid-liquid micro-extraction technology are combined, so that the endocrine disrupter extracting efficiency is high, the influence of impurities can be effectively removed, and quick and accurate qualitative and quantitative analysis on the trace endocrine disrupter can be realized.

Description

technical field [0001] The invention belongs to the technical field of extraction, and in particular relates to an extraction method of endocrine disruptors. Background technique [0002] For nearly half a century, reports on the decline in the quality of human reproductive health and abnormal reproduction in wild animals have increased year by year: decreased sperm count and motility in men, increased incidence of breast cancer in women; increased hermaphroditism in wild cockroaches living downstream of sewage treatment plants in the UK Abnormal gonad development and sex hormone levels in crocodiles in Florida, USA; Feminization of male fish in polluted rivers, increased hermaphroditism, etc. People gradually realize that there are some compounds in the environment that can simulate hormone effects, regulate or interfere with the function of the biological endocrine system, namely endocrine disruptors (EDCs), which are defined as "can interfere with the maintenance of biolo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/34
Inventor 康海宁原珂栾天罡岳振峰王晓玮叶刚林黎涂小珂
Owner FOOD INSPECTION CENT OF CIQ SHENZHEN
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com