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Method for extracting and separating multiple active constituents from polygonatum

An active ingredient, Polygonatum chinensis technology, applied in the field of Polygonatum chinensis extraction, can solve the problems of large consumption of organic solvents, complex process, large amount of organic solvents, etc., and achieve the effects of less process, simple equipment and less toxicity

Inactive Publication Date: 2015-09-30
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned polygonatum polysaccharide extraction process has the disadvantages of long time, high cost, complicated process and low polysaccharide purity.
Chinese patent CN 103788224 discloses a method for extracting Polygonatum polysaccharides from Chinese medicine Rhizoma Rhizoma Rhizoma Rhizoma Polygonatum, which adopts a process route of microwave-assisted extraction-concentration-ultrasonic oscillation-removing protein-passing column, and obtains Polygonatum polysaccharides through repeated separation and purification. This process has complex processes , long process time, large consumption of organic solvents, etc.
Wang Wenjun and others publicly published the extraction method of Polygonatum flavonoids, which adopts the process route of degreasing-ultrasonic-assisted extraction-decolorization-drying, the process is complicated, the amount of organic solvent required is large, the time is long, and the extraction rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Accurately weigh 4 grams of raw Polygonatum medicinal powder and mix it with 40ml50% ethanol, extract 0.5min with flash extraction technology, and suction filter to obtain the total saponins extract of Polygonatum and Rhizoma Polygonatum dregs; The evaporator is recovered to obtain the crude product of Polygonatum total saponins; the crude product of Polygonatum total saponins is diluted to a concentration of 0.8 mg / ml with distilled water, added to the prepared macroporous resin for adsorption, and first eluted with distilled water until it flows out Liquid does not show Molish reaction till, then use the ethanol that volume concentration is 70%, carry out elution, until the eluent has no saponin, consider elution is complete, after evaporating ethanol, vacuum freeze-dry to constant weight, obtain the endogenous saponin finished product; The yield of Polygonatin saponin was 6.85%, and the purity was 81.21%.

Embodiment 2

[0037]Accurately weigh 4 grams of raw Polygonatum medicinal material powder and mix with 40ml 65% ethanol, extract 1min with flash extraction technology, and suction filter to obtain the total saponins extract of Polygonatum and Rhizoma Polygonatum dregs; The evaporator is recovered to obtain the crude product of Polygonatum total saponins; the crude product of Polygonatum total saponins is diluted to a concentration of 0.9 mg / ml with distilled water, added to the prepared macroporous resin for adsorption, and first eluted with distilled water until it flows out Liquid does not show Molish reaction till, then use the ethanol that volume concentration is 75%, carry out elution, until eluent has no saponin, consider elution is complete, after evaporating ethanol, vacuum freeze-dry to constant weight, obtain the finished product of Polygonatin saponin; The yield of Polygonatin saponin was 6.89%, and the purity was 79.53%.

Embodiment 3

[0039] Accurately weigh 4 gram of Rhizoma Rhizoma Rhizoma Polygonatum Medicinal Material powder and mix with 40ml 80% ethanol, extract 1.5min with flash extraction technique, extract twice, combine extract, suction filtration, obtain Rhizoma Rhizoma Polygonatum total saponin extract and Rhizoma Rhizoma Polygonatum dregs; The ethanol in the Polygonatum total saponins extract is reclaimed with a rotary evaporator to obtain the Polygonatum total saponins crude product; the Polygonatum total saponins crude product is diluted to a concentration of 1.0mg / ml with distilled water, added to the prepared macroporous resin for adsorption, first Elute with distilled water until the effluent does not show Molish reaction, and then use ethanol with a volume concentration of 80% to elute until the eluate is free of saponin. To constant weight, the finished product of Polygonatin saponin is obtained; the yield of the obtained Polygonatin saponin is 6.95%, and the purity is 79.49%.

[0040] (2...

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Abstract

The invention discloses a method for extracting and separating multiple active constituents from polygonatum. Active constituents including polygahatous polysaccharides, polygahatous saponin and polygahatous flavones are extracted and separated through the homogenate extraction technique. Compared with traditional methods, the method has the advantages that extraction condition is mild, and efficient constituents are prevented from being damaged under heating; solvent consumption is low, extraction is quick and efficient, material extraction can be completely achieved in 0.5-1.5 min, and extraction rate is high; separation and purification are further conducted through macroporous resin, so that purification is effectively improved; wherein, the yield and purity of polygahatous polysaccharides are 28.37% and 88.76% respectively, the yield and purity of polygahatous saponin are 4.95% and 89.49% respectively, and the yield and purity of polygahatous flavones are 1.97% and 82.81% respectively. Polygahatous saponin and polygahatous flavones byproducts are extracted through the homogenate extraction technique so that the polygahatous polysaccharides can be extracted, polygahatous medicinal material resources are saved, the effective extraction rate and comprehensive utilization rate of multiple active constituents of polygonatum are increased, and resource waste is avoided.

Description

technical field [0001] The invention belongs to a method for extracting Polygonatum, in particular to a method for extracting Polygonatum total saponins, Polygonatum total flavonoids and Polygonatum polysaccharides. Background technique [0002] Polygonatum is a perennial herb of the Liliaceae Polygonatum Mill. In recent years, domestic and foreign studies on Polygonatum plants have continued to deepen, and various active components such as polysaccharides, saponins, and flavonoids have been isolated from this genus. Existing studies have shown that Polygonatum polysaccharides have the effects of enhancing immune function, anti-tumor, anti-radiation, enhancing memory, and anti-fatigue; the biological activity research of Polygonatum saponins is mostly focused on lowering blood sugar, anti-tumor, and anti-HIV; and Polygonatum flavonoids also have A wide range of biological activities, including eliminating fatigue, protecting blood vessels, preventing atherosclerosis, loweri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8969
Inventor 杨占东袁诚李金华陈路林罗明琍钟鸣曾荣华李益
Owner GUANGZHOU HANFANG PHARMA
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