SPN locus in linkage with round or flat character of peach fruit shape, molecular marker based on locus and application thereof

Abstract

The invention discloses an SPN locus in linkage with the round or flat character of a peach fruit shape, a molecular marker based on the locus and application thereof. The SPN locus is located on the position of 2453bp of gene PPa003772 mg, and the sequence coding direction is in the 5'-3' direction. The SPN locus is exactly located on the position of the last 3' end basic group of downstream R primer marked down by an AS-PCR molecule, meanwhile, a 3' end secondary basic group is subjected to mispairing and changed into guanine deoxyribonucleotide G from thymine deoxyribonucleotide T. Through the method, in the flat peach and common peach seed selection work, the workload of peach tree seed breeding can be effectively reduced, the seed breeding time is shortened, and the seed breeding efficiency is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a SNP locus linked with the round and flat traits of peach fruit, a molecular marker based on the locus and an application thereof. Background technique [0002] Peach is a perennial deciduous fruit tree of the subgenus Prunus in the Rosaceae family. Its fruit is soft and juicy, and has a pleasant flavor. It is a popular fruit. Peach fruit has high nutritional value. It is rich in multi-candy pectin and contains various vitamins and mineral elements. It is a fruit suitable for all ages. [0003] At present, peach fruits are mainly divided into two types in terms of fruit shape: flat flat peaches and round common peaches. Each type has a corresponding consumer group and important utilization value, but for today's peach industry system, there are still few varieties with good quality. Cultivating high-quality flat peaches and ordinary peaches has become an urgent probl...

AI Technical Summary

Problems solved by technology

[0004] At present, breeders mainly use the traditional method of cross breeding new varieties in peach breeding, which has a long breeding cycle, a large area, time-consuming and labor-intensive, and low breeding efficiency

This is especially true for the breeding of ordinary peaches and flat peaches. It is impossible to distinguish the traits of ordinary peaches and flat peaches in the early breeding.

[0005] Moreover, there is no effective and accurate molecular marker in the peach tree breeding work for the selection of common peaches and flat peaches. Breeders can only wait until the trees reach the ability to bloom and bear fruit, and thus the work of cultivating common peaches and flat peaches costs a lot. Long time, heavy workload and low efficiency

[0006] However, with the continuous improvement of consumer demand, how to quickly and efficiently breed common peach and flat peach varieties with better flavor and better quality has become a severe challenge for breeders.

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