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Method and system for extracting and separating flavone glycoside monomer from Desmodium styracifolium

A technology of Radix vulgaris and its extract is applied in the field of phytochemistry and achieves the effects of short production time, high product yield and simple process operation.

Active Publication Date: 2015-10-14
WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods and systems for extracting and separating this compound need to be improved urgently

Method used

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  • Method and system for extracting and separating flavone glycoside monomer from Desmodium styracifolium
  • Method and system for extracting and separating flavone glycoside monomer from Desmodium styracifolium
  • Method and system for extracting and separating flavone glycoside monomer from Desmodium styracifolium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Figure 4 A general scheme of the method of the invention is shown.

[0032] 1. Raw materials: select 45000-50000 parts by weight of the leguminous plant Desmodium glabrata, cut into 5-10cm sections, wash off the sediment with drinking water, drain and feed.

[0033] 2. Ethanol extraction: reflux extraction with 80% ethanol twice, the first time 12 times the amount for 2 hours, the second time 10 times the amount for 1.5 hours; combine the two alcohol extracts, and recover the ethanol until it has no alcohol smell.

[0034] 3. Concentration under reduced pressure: add water to dilute the extract concentrate to 5 times the volume of the medicinal material; filter it with a 200-mesh filter cloth; get the column liquid; pack the net product resin that has been treated with 95% ethanol in advance, and replace it with purified water; The upper column liquid is pumped into the column for adsorption, and the flow rate is controlled to be 0.5-1 times BV / h. After the adsorption...

Embodiment 2

[0047] 1. Carry out steps 1-3 of Example 1.

[0048] 2. Multi-stage chromatographic separation

[0049] (1) 300-400 parts by weight of Guangjin crude drug is separated by AB-8 macroporous resin chromatography, that is, chromatographic separation I, ethanol-water mixed solvent 25:75, 35:65, 45:55, 65:35 gradient washing Take off, wash each gradient until there is no color, combine the fractions to get 25%, 35%, 45%, and 65% in four parts;

[0050] (2) Use ODS reversed-phase medium-pressure chromatography to separate 65% of the part obtained by chromatographic separation I, that is, chromatographic separation II, use ethanol-water, the condition is 20% isocratic for 30 minutes, and 20-40% gradient is eluted for 200 minutes, according to the chromatographic Figure out the peak sequence, collect in fractions, collect one fraction per 100-150 volume fractions, collect 40-50 fractions in total, and merge the same fractions after HPLC detection;

[0051] (3) Combine the 36-50 fract...

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Abstract

The invention provides a method for extracting and separating a genistein-7-O-beta-D-apiofuranosyl-(1->6)-O-beta-D-glucopyranoside compound shown as formula 1. The method comprises the following steps: utilizing ethyl alcohol to perform reflux extraction on Desmodium styracifolium so as to obtain a Desmodium styracifolium extractive; and utilizing alcohol systems to perform multistage chromatographic separation on the extractive, including utilizing a first alcohol system to perform first chromatographic separation on the extractive so as to obtain a first separation part, utilizing a second alcohol system to perform second chromatographic separation on the first separation part so as to obtain a second separation part, and utilizing a third alcohol system to perform third chromatographic separation on the second separation part so as to obtain the compound shown as the formula 1. The compound shown as the formula 1 is separated and extracted from the Desmodium styracifolium by utilizing the method provided by the invention, the technology is simple, the production time is short, the yield is high, the purity is high, and the compound shown as the formula 1 with the purity of more than 95% can be obtained.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and relates to a method and a system for extracting and separating flavonoid oxyglycoside monomers from Desmodium sativa Methods and systems for genistein-7-O-β-D-apifuranosyl-(1→6)-O-β-D-glucopyranoside. Background technique [0002] Desmodium styracifolium (Osbeck) Merr. is a plant of the leguminous genus Desmodium styracifolium (Osbeck) Merr. Its medicinal part is the dry aerial part, and its main chemical components are flavonoids, saponins, polysaccharides, alkaloids and other compounds. It has the effects of clearing away heat and dehumidification, diuresis and treating stranguria. It is used for hot shower, sand shower, stone shower, astringent and painful urination, edema and oliguria, jaundice, red urine, and urinary calculi. [0003] Genistein-7-O-β-D-apifuranosyl-(1→6)-O-β-D-glucopyranoside, its chemical structure is shown in Formula 1, it is a yellow powder, and its molecular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 王学海许勇李莉娥杨仲文余通冯芸尹海龙杨婷黄璐曹儒宾谢长
Owner WUHAN OPTICS VALLEY HUMANWELL BIO PHARMA