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Method of preparing linoleate isomerase micro-capsules on the basis of polyelectrolyte layer-by-layer self-assembly technology

A technology of linoleic acid isomerase and layer-by-layer self-assembly, which is applied in the same direction fixed on/in the organic carrier to achieve the effect of controllable wall thickness and high specific surface area

Inactive Publication Date: 2015-10-14
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using traditional methods to immobilize enzymes can improve the catalytic efficiency, stability and half-life of enzymes, etc. These methods have their own advantages and disadvantages, and they need to be selected according to actual needs

Method used

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  • Method of preparing linoleate isomerase micro-capsules on the basis of polyelectrolyte layer-by-layer self-assembly technology
  • Method of preparing linoleate isomerase micro-capsules on the basis of polyelectrolyte layer-by-layer self-assembly technology
  • Method of preparing linoleate isomerase micro-capsules on the basis of polyelectrolyte layer-by-layer self-assembly technology

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] Example 1: Effect of temperature on enzymatic reactions

[0028] The linoleic acid isomerase microcapsules and free linoleic acid isomerase were reacted at 20, 30, 40, 50, 60, 70, and 80°C respectively, and the enzyme specific activity was determined; the highest enzyme specific activity is 100%, the ratio of the enzyme specific activity under other temperatures to the highest enzyme specific activity is the relative specific activity, and the relative specific activity of linoleic acid isomerase microcapsules and free linoleic acid isomerase is shown in Table 1 under each temperature condition and figure 1 shown.

[0029] The influence of table 1 temperature on enzymatic reaction

[0030] Tab.1Effect of temperature on the enzymatic reaction

[0031]

[0032] From Table 1 and figure 1 It can be seen that the optimum reaction temperature of free linoleic acid isomerase is 40°C, the optimum reaction temperature of linoleic acid isomerase microcapsules is 50°C, and ...

example 2

[0033] Example 2: Comparison of thermal stability of linoleic acid isomerase microcapsules and free linoleic acid isomerase

[0034] Incubate linoleic acid isomerase microcapsules and free linoleic acid isomerase at 20, 30, 40, 50, 60, 70, and 80°C for one hour, and then carry out at 37°C after the incubation. Enzyme-catalyzed reaction, measure the enzyme specific activity; take the highest enzyme specific activity as 100%, the ratio of the enzyme specific activity at other temperatures to the highest enzyme specific activity is the relative specific activity, the linoleic acid isomerase microcapsules under each heat preservation condition and the relative specific activity of free linoleic acid isomerase as table 2 and figure 2 shown.

[0035] Table 2 Comparison of thermal stability of linoleic acid isomerase microcapsules and free linoleic acid isomerase

[0036] Tab.2 Comparison of the thermal stability of the microcapsule and free LAI

[0037]

[0038] From Table 2 ...

example 3

[0039] Example 3: Effect of pH on Enzymatic Reactions

[0040] The linoleic acid isomerase microcapsules and free linoleic acid isomerase were respectively reacted under the pH conditions of 2, 3, 4, 5, 6, 7, 8, 9, and 10, and the enzyme specific activity was determined; The enzyme specific activity is 100%. The ratio of the enzyme specific activity at other temperatures to the highest enzyme specific activity is the relative specific activity. The relative specific activity of linoleic acid isomerase microcapsules and free linoleic acid isomerase under various pH conditions as table 3 and image 3 shown.

[0041] The influence of table 3pH on enzymatic reaction

[0042] Tab.3Effect of pH on the enzymatic reaction

[0043]

[0044] From Table 3 and image 3 It can be seen that the optimum reaction pH of free linoleic acid isomerase is 4, the optimum reaction pH of linoleic acid isomerase microcapsules is 5, and the optimum reaction pH of microencapsulated linoleic acid ...

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Abstract

The invention provides a method of preparing linoleate isomerase micro-capsules on the basis of a polyelectrolyte layer-by-layer self-assembly technology and can solve a problem that free linoleate isomerase is poor in tolerance, is poor in stability and cannot be recycled in industrial production. The method of the invention includes the following steps: with an amorphous calcium carbonate crystal, which is obtained through co-precipitation of calcium carbonate and the linoleate isomerase, as a template and with polyallylamine hydrochloride and sodium polystyrenesulfonate as coating materials, preparing the linoleate isomerase micro-capsules through the polyelectrolyte layer-by-layer self-assembly technology. The micro-capsule is high in activity, excellent in stability and long in half life.

Description

Technical field: [0001] The invention belongs to the technical field of enzyme engineering research and development. The invention relates to the research on a method for preparing linoleic acid isomerase microcapsules by polyelectrolyte layer-by-layer self-assembly technology. Background technique: [0002] Linoleic acid isomerase (LAI) is an isomerase that can specifically convert linoleic acid into conjugated linoleic acid. As conjugated linoleic acid with strong physiological functions has attracted widespread attention, as a key enzyme in the process of bioconverting linoleic acid into conjugated linoleic acid, linoleic acid isomerase has become a research hotspot. . At present, free linoleic acid isomerase has problems such as easy inactivation, easy agglomeration, low tolerance to environmental changes, difficult separation from products, and non-recyclability during industrial application, which reduces the catalytic activity of the enzyme. Efficiency, but also in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/04
Inventor 赵国芬张颜婷张峰贾丽李晨曦
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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