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Leymus chinensis triosephosphate isomerase gene as well as encoding protein and application thereof

A technology of triose phosphate and encoded protein, applied in the direction of isomerase, application, genetic engineering, etc., can solve the problem of low stress resistance of plants and achieve the effect of improving stress resistance

Inactive Publication Date: 2015-10-14
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low stress resistance of plants, and to provide a gene of Leymus chinensis triose phosphate isomerase and its encoded protein and its application

Method used

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  • Leymus chinensis triosephosphate isomerase gene as well as encoding protein and application thereof
  • Leymus chinensis triosephosphate isomerase gene as well as encoding protein and application thereof
  • Leymus chinensis triosephosphate isomerase gene as well as encoding protein and application thereof

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specific Embodiment approach 1

[0019] Specific embodiment 1: The nucleotide sequence of Leymus chinensis triose phosphate isomerase gene in this embodiment is shown in SEQ ID No: 1.

[0020] In this embodiment, the Leymus chinensis triose phosphate isomerase gene consists of 1053 bases, including the 5' end untranslated region (1-77), the protein coding region (78-839) and the 3' end untranslated region (840 -1053).

[0021] In this embodiment, the Leymus chinensis triose phosphate isomerase gene is obtained according to the following method:

[0022] 1. Use the RNA extraction kit to extract the total RNA in Leymus chinensis, use the TOYOBO ReverTra Ace-α-kit to reverse-transcribe it into cDNA, and perform PCR amplification with the following primers:

[0023] f 1 , 5'-CGGTGAAGTCAGTGCTGAGATGC-3';

[0024] R 1 , 5'-GCTCCACCAACGAGGAAACCATC-3';

[0025] Recover and purify the PCR product to obtain the conserved region sequence of the LcTPI gene;

[0026] 2. The total RNA of Leymus chinensis in step 1 was...

specific Embodiment approach 2

[0031] Specific embodiment 2: The amino acid sequence of the protein encoded by the triose phosphate isomerase gene of Leymus chinensis in this embodiment is shown in SEQ ID No.2.

[0032] In this embodiment, the encoded protein of Leymus chinensis triose phosphate isomerase gene consists of 253 amino acids, the molecular weight is 26.9KD, and the isoelectric point is 5.57.

specific Embodiment approach 3

[0033] Specific Embodiment Three: Application of Leymus chinensis triose phosphate isomerase gene in this embodiment: Leymus chinensis triose phosphate isomerase gene is introduced into target plants to cultivate transgenic plants with enhanced stress resistance.

[0034] In this embodiment, the target plant is a dicotyledonous plant or a monocotyledonous plant; the stress resistance is resistance to NaCl and methylglyoxal.

[0035] Adopt the following examples to verify the beneficial effects of the present invention:

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Abstract

The invention relates to a Leymus chinensis triosephosphate isomerase gene as well as an encoding protein and application thereof, relates to the field of biotechnology, and aims to solve the problem of low stress resistance of plants. The nucleotide sequence of the Leymus chinensis triosephosphate isomerase gene is shown in SEQ ID No:1. The amino acid sequence of the encoding protein of the Leymus chinensis triosephosphate isomerase gene is shown in SEQ ID No:2. The application of the Leymus chinensis triosephosphate isomerase is as follows: the Leymus chinensis triosephosphate isomerase gene is introduced to a target plant for culturing to obtain a transgenic plant with enhanced stress resistance. The expression of the LcPTI gene provided by the invention is induced by neutral salt, basic salt or PEG-simulated drought, and the LcPTI gene can participate in the response of Leymus chinensis to multiple environmental stresses, so that the stress resistance of the plant is enhanced. The introduction of the LcPTI gene to the target plant for culturing to obtain the transgenic plant with enhanced stress resistance has significance to cultivation of plant variety with enhanced stress resistance, in particular resistance to methyl-glyoxal and NaCl.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a gene of Leymus chinensis triose phosphate isomerase, its encoded protein and its application. Background technique [0002] Global crop yield reduction is mainly caused by abiotic stress factors such as salinity, drought, heavy metal poisoning, and extreme temperature. All these adversity stresses will lead to the imbalance of the body's metabolism, causing excessive accumulation of methylglyoxal (MG) and reactive oxygen species (ROS) in the cells, causing secondary oxidative stress to plants. Regarding the anti-oxidative stress effect, more attention has been paid to the scavenging of ROS by antioxidant enzymes and non-enzymatic antioxidants in the past. However, under adversity stress, MG in the body is an important reason for the massive outbreak of ROS, and the metabolic pathways and clearance systems of ROS and MG in the body are closely related. MG is an active carbonyl sub...

Claims

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Application Information

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IPC IPC(8): C12N15/61C12N9/90C12N15/82A01H5/00
Inventor 崔继哲刘欣卢倩赵春梅李若男张宝懿孟雪
Owner HARBIN NORMAL UNIVERSITY
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