Method for enriching GABA (gamma-aminobutyric acid) in mulberry leaves

Abstract

The invention relates to a method for enriching GABA (gamma-aminobutyric acid) in mulberry leaves. The method comprises the steps that: ganoderma lucidum is activated by a PDA (Potato-Dextrose-Agar) culture plate, and then is transferred to a PDA liquid culture medium to be cultured until mycelial pellets grow from the culture which is used as a fermentation seed; according to the inoculum size of 1-3% of volume ratio, the cultivated culture is inoculated into a mulberry leaf culture medium, and after shaking and fermentation at constant temperature, a mulberry leaf ganoderma lucidum fermentation broth with high content of GABA is obtained. The mulberry leaf culture medium comprises mulberry leaf powder with mass percent of 1-5%, 0.1-0.5 mg / 100ml calcium chloride, and a PDA liquid culture medium with pH being 6-8. The method disclosed by the invention is simple and convenient to operate, the yield is high, no pollution is generated, and separation and purification are facilitated. The mulberry leaf fermentation broth with high content of GABA can be further developed into functional food, which has a pharmacological function of preventing and treating geriatric diseases and mental diseases, and higher application value is realized.

Description

technical field [0001] The invention belongs to the field of fermentation engineering and production technology of food functional components, and in particular relates to a method for increasing GABA content in mulberry leaves. Background technique [0002] γ-aminobutyric acid (GABA for short, also known as aminobutyric acid), is a non-protein functional amino acid, an important inhibitory neurotransmitter in the central nervous system of mammals, and has important physiological functions: regulating blood pressure and heart rate , nourish nerve cells, treat epilepsy, promote mental stability, promote brain blood flow, enhance brain vitality, promote growth hormone secretion, assist in the treatment of asthma, activate liver and kidney functions, prevent obesity, promote alcohol metabolism, improve mental disorders in early aging and menopause Syndrome and many other effects. The Ministry of Health officially issued a document on September 27, 2009 to list GABA as a new re...

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Problems solved by technology

Few scholars at home and abroad use mulberry leaves to enrich γ-aminobutyric acid

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