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Accurate and stable total cholesterol detection reagent

A technology for total cholesterol and detection reagents, applied in biological testing, material inspection products, etc., can solve problems such as poor accuracy, red-purple pigment instability, performance defects, etc., and achieve good accuracy and stability

Inactive Publication Date: 2015-10-14
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the actual research and testing process, we will find that in the third step of the enzymatic reaction, that is, reaction (3), due to the instability of the product red-purple pigment, the test result of total cholesterol is unstable and the accuracy is poor
At present, the enzymatic detection kits circulating in the market generally have this performance defect

Method used

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  • Accurate and stable total cholesterol detection reagent
  • Accurate and stable total cholesterol detection reagent
  • Accurate and stable total cholesterol detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Reagent R

[0027] MOPSO buffer (pH7.0) 50mmol / L

[0028] 4-Aminoantipyrine 0.5mmo1 / L

[0029] Cholesterol esterase (CHE) 2KU / L

[0030] Cholesterol oxidase (CHO) 2KU / L

[0031] Peroxidase (POD) 2KU / L

[0032] Sodium 3,5-dichloro-2-hydroxybenzenesulfonate (DHBS) 2mmol / L

[0033] Magnesium chloride 2mmol / L

[0034] Trehalose 10 g / L

[0035] Fatty alcohol polyoxyethylene ether (AEO-9) 1 0g / L

[0036] The operation is as shown in Table 1:

[0037] Table 1 Detection method of total cholesterol detection reagent

[0038]

[0039] Calculation: total cholesterol content (mmo1 / L) = (determination / min÷standard / min)×C standard.

Embodiment 2

[0041] Reagent R

[0042] MOPSO buffer (pH7.0) 50mmol / L

[0043] 4-Aminoantipyrine 0.5mmo1 / L

[0044] Cholesterol esterase (CHE) 1KU / L

[0045] Cholesterol oxidase (CHO) 1KU / L

[0046] Peroxidase (POD) 1KU / L

[0047] Sodium 3,5-dichloro-2-hydroxybenzenesulfonate (DHBS) 2mmol / L

[0048] Magnesium chloride 2mmol / L

[0049] Trehalose 1 g / L

[0050] Fatty alcohol polyoxyethylene ether (AEO-9) 1 g / L

[0051] The detection steps are as in Example 1.

[0052] Validation of the accuracy of a stable total cholesterol detection reagent

[0053] For the same high-value serum sample, the concentration of total cholesterol is about 200 mg / dL. This sample is diluted with normal saline, and the gradient is shown in Table 2.

[0054] Table 2

[0055]

[0056] Utilize respectively embodiment 1 and embodiment 2 reagent to detect each concentration gradient sample, the result is as follows figure 1 As shown, Example 2 changes linearly with the dilution concentration, and the ...

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Abstract

The present invention provides an accurate and stable total cholesterol detection reagent, which comprises: 50 mmol / L of a MOPSO buffer, 0.1-1.0 mmo1 / L of antipyrine, 1-2 KU / L of cholesterol esterase, 1-2 KU / L of cholesterol oxidase, 1-2 KU / L of peroxidase, 2-5 mmol / L of sodium 3,5-dichloro-2-hydroxybenzene sulfonate, 2-5 mmol / L of magnesium chloride, 1-10 g / L of trehalose, and 1-10 g / L of fatty alcohol polyoxyethylene ether. The detection result of the reagent of the present invention has good accuracy and good stability.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an accurate and stable in vitro detection reagent for total cholesterol content. Background technique [0002] Total cholesterol refers to the sum of cholesterol contained in all lipoproteins in the blood. It is an important indicator of clinical blood lipid analysis. Plasma cholesterol mainly exists in low-density lipoprotein (LDL), followed by high-density lipoprotein (HDL) and very low-density lipoprotein. protein (VLDL), the least content in chylomicrons (CM). In human plasma, cholesterol exists in two forms, cholesteryl ester and free cholesterol. The former accounts for about 70% and the latter accounts for 30%. [0003] Cholesterol contained in plasma and tissues is often in a state of exchange, and the exchange rate varies with different tissues. Since cholesterol continuously enters and exits the blood, plasma cholesterol not only refle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 甘宜梧张永英李静赵新王春艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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