Making method of rose apple enzyme health care beverage
A technology of health drink and rose apple enzyme, which is applied in the field of bioengineering, can solve the problems that the enzyme activity cannot be retained for a long time, cannot guarantee the absence of pathogenic bacteria, and the fermentation time is long, so as to inhibit the growth of miscellaneous bacteria, retain enzyme activity, and maintain stable quality Effect
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Embodiment 1
[0024] A method for fermenting rose apple ferment, comprising the following steps:
[0025] (1) Select ripe rose apples with fresh color and high natural enzyme content as the main raw material for fermentation.
[0026] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 10% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0027] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0028] (4) According to the inoculation ratio of 5%, the nectar-derived bacteria and lactobacillus were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.
[0029] (5) Coarse filtration is carried out fi...
Embodiment 2
[0031] A method for fermenting rose apple ferment, comprising the following steps:
[0032] (1) Select ripe apples with fresh color and high natural enzyme content as the main raw material for fermentation.
[0033] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 40% of the volume, add 15% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0034] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0035] (4) According to the inoculation ratio of 4%, the nectar-derived bacteria and lactic acid bacteria were respectively taken and inserted into the fermentation stock solution, and fermented for 10 days, with a pH value of 6.5 and a temperature of 28°C.
[0036] (5) Coarse filtration is carried out...
Embodiment 3
[0038] A method for fermenting rose apple ferment, comprising the following steps:
[0039] (1) Select ripe apples with fresh color and high content of natural enzymes as the main raw material for fermentation.
[0040] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 50% of the volume, add 20% of the total liquid volume % of the raw honey is for the fermentation stock solution.
[0041] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0042] (4) According to the inoculation ratio of 3%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted into the fermentation stock solution, and fermented for 14 days, with a pH value of 7.0 and a temperature of 25°C.
[0043] (5) Coar...
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