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Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation

A technology of sodium gluconate and medium sterilization, applied in the direction of fermentation, can solve problems such as large steam consumption, and achieve the effects of improving fermentation efficiency, reducing residual sugar and improving quality

Active Publication Date: 2015-10-28
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture medium sterilization process needs to heat all the materials to 121 ° C and maintain it for a certain period of time, and the steam consumption is relatively large (see the appendix for the process) figure 1 )

Method used

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  • Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation
  • Method for sterilization of medium for production of sodium gluconate by Aspergillus niger fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 300m 3 Fermentation Medium Continuous Sterilization Technology

[0027] 1) Dilute the starch milk with water to a mass concentration of 32.8% (w / v), adjust the pH value to 5.5, add liquefaction enzyme in proportion, heat up to 105-108°C instantaneously through the injector, enter the maintenance tank, and maintain at 105°C for 5 minutes , and then enter the medium temperature maintenance tank through the flash tank, maintain 1h-1.5h at 95°C-98°C, then heat up to 125°C-130°C through secondary spraying, maintain 125°C for 5min, and cool the material to 62°C through a heat exchanger , enter the saccharification tank, add acid to adjust the pH to 4.5, add saccharification enzyme preparation in proportion, and keep saccharification at 60°C-62°C until the saccharification is completed.

[0028] 2) After the saccharification is completed, the saccharification liquid is filtered through a filter, and the obtained clarified sugar liquid is pumped to the batching tank....

Embodiment 2

[0034] Example 2 300m 3 Basic invention of fermentation culture Sterilization process

[0035] 1) Add water to the commercial starch or dilute the starch milk to a mass concentration of 33.5% (w / v), adjust the pH value to 5.6, add liquefying enzyme in proportion, and heat up to 105-108°C instantaneously through the injector, then enter the maintenance process Tank, maintained at 105°C for 5 minutes, then enters the medium temperature maintenance tank through the flash tank, maintained at 95°C-98°C for 1h-1.5h, then raised the temperature to 125°C-130°C through secondary spraying, maintained at 128°C for 5min, passed through the heat exchanger Cool the material to 62°C, put it into a pre-sterilized saccharification tank, add acid to adjust the pH to 4.4, add glucoamylase preparations in proportion, and keep saccharification at 60°C-62°C until the end of saccharification.

[0036] 2) Steam is introduced into the connecting pipelines, valves, pipeline pumps, filters and fermente...

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PUM

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Abstract

The invention relates to a method for sterilization of a medium for production of sodium gluconate by Aspergillus niger fermentation. The method has the advantages that: (1) a prepared glucose solution is directly transported into a fermentation tank by an aseptic environment formed in a starch double-enzyme sugar making process, material mixing, continuous sterilization and other processes are omitted, over 95% of medium sterilization steam is saved, and the material mixing process is also simplified; and (2) after making of sugar from starch, the glucose solution is filtered and then directly transported into the fermentation tank without material mixing and sterilization processes, thus avoiding a high temperature treatment process, reducing the destruction of high temperature on glucose, and improving the feed liquid quality.

Description

technical field [0001] The invention relates to a method for sterilizing a fermentation medium, in particular to a method for sterilizing a medium for producing sodium gluconate by fermentation of Aspergillus niger. Background technique [0002] Sodium gluconate, also known as sodium pentahydroxycaproate, is a white or light yellow crystalline powder, easily soluble in water, slightly soluble in alcohol, insoluble in ether, and its molecular formula is C 6 h 11 o 7 Na, with a relative molecular weight of 218.14, sodium gluconate is a polyhydroxy organic acid salt with a wide range of uses, and is widely used in chemical, food, pharmaceutical and other industries. [0003] The production methods of sodium gluconate mainly include biological fermentation method and catalytic oxidation method, and the Aspergillus niger fermentation production process is generally used now. The process uses glucose as raw material, adding a small amount of inorganic salt and adding water to m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58
CPCC12P7/58
Inventor 田延军赵祥颖刘建军赵晨张家祥韩丽
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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