Carrageenan oligosaccharide with trepang growth promotion and adverse resistance functions and preparation method and application thereof
A carrageenan oligosaccharide and adverse action technology, which is applied in the field of microorganisms to achieve the effects of increasing growth rate, increasing stress resistance and immune function, and improving stress resistance.
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Embodiment 1
[0027] Example 1 Breeding of Carrageenan Oligosaccharide Degrading Bacteria (Cellulophaga lytica: M9)
[0028] 1. Screening of strains:
[0029] Take an appropriate amount of seaweed from the sea area of Haibei Square, Development Zone, Dalian City, Liaoning Province, and use sterile normal saline to carry out gradient dilution, and then 10 -2 ~10 -6 Spread the diluted solution on the plate, place the coated plate in a constant temperature incubator at 30°C for culture, observe the plate after 48-60 hours, pick a single colony with depressions or halos around it to optimize the conditions, and finally determine the experimental strain .
[0030] 2. Preparation of Carrageenan Oligosaccharides
[0031] (1) Preparation of crude enzyme solution
[0032] Inoculate the strain M9 into the liquid medium, and subculture with 2% inoculum after shaking for 16-20 hours. Shake the culture on an air bath shaker at 35°C with a rotation speed of 120r / min. After the strain is stable, tak...
Embodiment 2
[0037] The influence of embodiment 2 carrageenan oligosaccharides on sea cucumber growth
[0038] 1. Feed the sea cucumber with the prepared carrageenan oligosaccharide powder in combination with the sea cucumber basal feed, and raise the sea cucumber for 8 weeks. Feed early, change water late, monitor water temperature and room temperature in real time, and give oxygen 24 hours a day. The experiment was divided into five groups, and the oligosaccharides were added to the basal feed in proportions of 0, 0.5%, 2.5%, 5%, and 10%. The weight of the sea cucumber is between 3.75±0.20g+, and it is fed once a day, and the feeding amount is 3-5% of the weight of the sea cucumber. Change the water for sea cucumbers once a day, and clean up residual bait and feces. Real-time observation of water temperature and room temperature, observation of sea cucumber status, feeding and defecation.
[0039] 2. Effect of carrageenan oligosaccharides on the stress resistance of sea cucumbers
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