Carrageenan oligosaccharide with trepang growth promotion and adverse resistance functions and preparation method and application thereof

A carrageenan oligosaccharide and adverse action technology, which is applied in the field of microorganisms to achieve the effects of increasing growth rate, increasing stress resistance and immune function, and improving stress resistance.

Active Publication Date: 2015-11-04
DALIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the preparation of carrageenan oligosaccharides with g

Method used

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  • Carrageenan oligosaccharide with trepang growth promotion and adverse resistance functions and preparation method and application thereof
  • Carrageenan oligosaccharide with trepang growth promotion and adverse resistance functions and preparation method and application thereof
  • Carrageenan oligosaccharide with trepang growth promotion and adverse resistance functions and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Breeding of Carrageenan Oligosaccharide Degrading Bacteria (Cellulophaga lytica: M9)

[0028] 1. Screening of strains:

[0029] Take an appropriate amount of seaweed from the sea area of ​​Haibei Square, Development Zone, Dalian City, Liaoning Province, and use sterile normal saline to carry out gradient dilution, and then 10 -2 ~10 -6 Spread the diluted solution on the plate, place the coated plate in a constant temperature incubator at 30°C for culture, observe the plate after 48-60 hours, pick a single colony with depressions or halos around it to optimize the conditions, and finally determine the experimental strain .

[0030] 2. Preparation of Carrageenan Oligosaccharides

[0031] (1) Preparation of crude enzyme solution

[0032] Inoculate the strain M9 into the liquid medium, and subculture with 2% inoculum after shaking for 16-20 hours. Shake the culture on an air bath shaker at 35°C with a rotation speed of 120r / min. After the strain is stable, tak...

Embodiment 2

[0037] The influence of embodiment 2 carrageenan oligosaccharides on sea cucumber growth

[0038] 1. Feed the sea cucumber with the prepared carrageenan oligosaccharide powder in combination with the sea cucumber basal feed, and raise the sea cucumber for 8 weeks. Feed early, change water late, monitor water temperature and room temperature in real time, and give oxygen 24 hours a day. The experiment was divided into five groups, and the oligosaccharides were added to the basal feed in proportions of 0, 0.5%, 2.5%, 5%, and 10%. The weight of the sea cucumber is between 3.75±0.20g+, and it is fed once a day, and the feeding amount is 3-5% of the weight of the sea cucumber. Change the water for sea cucumbers once a day, and clean up residual bait and feces. Real-time observation of water temperature and room temperature, observation of sea cucumber status, feeding and defecation.

[0039] 2. Effect of carrageenan oligosaccharides on the stress resistance of sea cucumbers

[...

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Abstract

The invention provides carrageenan oligosaccharide with the trepang growth promotion and adverse resistance functions and a preparation method and application thereof, and relates to the field of microorganisms. The carrageenan oligosaccharide obtained through the method can be used for food, feed additives, health care products, biopesticide and the like, and the application range is wide. The carrageenan oligosaccharide is used as feed additives for feeding trepang, the trepang growth condition is better, thorns are strong, the trepang moves freely, the ingestion force is strong, and the carrageenan oligosaccharide has the obvious promotion effect on trepang growth, can improve the trpang adverse resistance, improves the stress resistance and immune function of the trepang, and is an ideal trepang bait additive.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a carrageenan oligosaccharide capable of promoting the growth of sea cucumbers and resisting stress, as well as its preparation method and application. Background technique [0002] Carrageenan is a marine polysaccharide extracted from red algae, which is mainly used in the alginate industry. It has high biological activities, such as anticoagulation, anti-virus, lowering cholesterol and anti-oxidation, but the solubility of the polysaccharide is not good. Well, it is difficult to absorb, which limits its application in the field of biomedicine. At present, the methods for degrading polysaccharides mainly include physical degradation, chemical degradation and biodegradation. Both chemical degradation and physical degradation are random degradation, and the molecular fragments of the obtained oligosaccharides are not uniform. Microorganisms can produce enzymes with unique physiolog...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23K1/16A23K1/18C12R1/01
Inventor 吴海歌姚子昂李倩崔红利仇哲
Owner DALIAN UNIVERSITY
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